Stable expression and integrated hepatitis B virus genome in a human hepatoma cell line

被引：4

作者：

Wang, L. Y. ^{[1
]}

Li, Y. G. ^{[2
]}

Chen, K. ^{[1
]}

Li, K. ^{[1
]}

Qu, J. L. ^{[1
]}

Qin, D. D. ^{[1
]}

Tang, H. ^{[1
]}

机构：

[1] Chongqing Med Univ, Affiliated Hosp 2, Minist Educ, Key Lab Mol Biol Infect Dis, Chongqing, Peoples R China

[2] Chongqing Med Univ, Dept Forens Med, Chongqing, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 02期

关键词：

Hepatitis B virus; Stable cell line; HepG2; cell; Hygromycin; IN-VITRO SYSTEM; SURFACE-ANTIGEN; INFECTION; PARTICLES; DNA;

D O I：

10.4238/2012.May.18.3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HepG2.2.15 cell is a widely used cell model for studying HBV (hepatitis B virus) in vitro. In these cells, the HBV genome is integrated in several sites of HepG2 cellular DNA. These multiple copies may have some influence on the cellular processes. We constructed a new plasmid, pSEH-Flag-HBV, and transfected it into HepG2 cells, and then screened it with hygromycin. We then used ELISA, PCR, and RT-PCR to detect the expression of HBV in these cell lines. A cell line that stably expressed hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) was established. Using Southern blotting analysis, we found that the HBV genome was integrated as a single copy in the cellular DNA. This cell line will be a useful alternative model for HBV studies.

引用

页码：1442 / 1448

页数：7

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