First report of Enterocytozoon bieneusi from dairy cattle in Argentina

被引：55
作者：
Del Coco, Valeria F. [1 ,2 ]
Alejandra Cordoba, Maria [1 ,3 ]
Bilbao, Gladys [4 ]
de Almeida Castro, Pinto [4 ]
Angel Basualdo, Juan [1 ]
Santin, Monica [5 ]
机构：
[1] Univ Nacl La Plata, Catedra Microbiol & Parasitol, Fac Ciencias Med, RA-1900 La Plata, Buenos Aires, Argentina
[2] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina
[3] Comis Invest Cient Prov Buenos Aires, La Plata, Buenos Aires, Argentina
[4] Univ Nacl Ctr Prov Buenos Aires, Fac Ciencias Vet, Buenos Aires, DF, Argentina
[5] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA
来源：
VETERINARY PARASITOLOGY | 2014年 / 199卷 / 1-2期
关键词：
Argentina; Cattle; Enterocytozoon bieneusi; Genotype; Microsporidia; PCR; GENOTYPE; MICROSPORIDIOSIS; PREVALENCE; IDENTIFICATION; CALVES;
D O I：
10.1016/j.vetpar.2013.09.024
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Fecal specimens were obtained from a total of 70 dairy calves less than two months old on 11 municipalities in Buenos Aires, Argentina. After removal of fecal debris by sieving and sucrose flotation, specimens were subjected to PCR to detect the presence of Enterocytozoon bieneusi. PCR revealed a 14.3% of prevalence for E. bieneusi with 10 positive calves from 7 municipalities. Gene sequence analysis conducted in all samples positives by PCR revealed the presence of six genotypes; four previously reported in cattle as well as humans (D, I, J, and BEB4), one never reported in cattle before but previously reported in humans (EbpC), and one novel genotype (BEB10). These results constitute the first molecular characterization of E. bieneusi in Argentina, and suggest a potential risk of zoonotic transmission in this area. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：112 / 115
页数：4
相关论文
共 20 条
[1]   Enterocytozoon bieneusi at the wildlife/livestock interface of the Kruger National Park, South Africa [J].
Abu Samra, Nada ;
Thompson, Peter N. ;
Jori, Ferran ;
Zhang, Hongwei ;
Xiao, Lihua .
VETERINARY PARASITOLOGY, 2012, 190 (3-4) :587-590
[2]   The epidemiology of intestinal microsporidiosis in patients with HIV/AIDS in Lima, Peru [J].
Bern, C ;
Kawai, V ;
Vargas, D ;
Rabke-Verani, J ;
Williamson, J ;
Chavez-Valdez, R ;
Xiao, LH ;
Sulaiman, I ;
Vivar, A ;
Ticona, E ;
Navincopa, M ;
Cama, V ;
Moura, H ;
Secor, WE ;
Visvesvara, G ;
Gilman, RH .
JOURNAL OF INFECTIOUS DISEASES, 2005, 191 (10) :1658-1664
[3]   Prevalence of Enterocytozoon bieneusi in swine:: an 18-month survey at a slaughterhouse in Massachusetts [J].
Buckholt, MA ;
Lee, JH ;
Tzipori, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (05) :2595-2599
[4]   Transmission of Enterocytozoon bieneusi between a child and guinea pigs [J].
Cama, Vitaliano A. ;
Pearson, Julie ;
Cabrera, Lilia ;
Pacheco, Luz ;
Gilman, Robert ;
Meyer, Sarah ;
Ortega, Ynes ;
Xiao, Lihua .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) :2708-2710
[5]   Microsporidiosis: current status [J].
Didier, Elizabeth S. ;
Weiss, Louis M. .
CURRENT OPINION IN INFECTIOUS DISEASES, 2006, 19 (05) :485-492
[6]   Microsporidiosis: not just in AIDS patients [J].
Didier, Elizabeth S. ;
Weiss, Louis M. .
CURRENT OPINION IN INFECTIOUS DISEASES, 2011, 24 (05) :490-495
[7]   First detection of microsporidia in dairy calves in North America [J].
Fayer, R ;
Santín, M ;
Trout, JM .
PARASITOLOGY RESEARCH, 2003, 90 (05) :383-386
[8]   Species of Cryptosporidium detected in weaned cattle on cow-calf operations in the United States [J].
Fayer, Ronald ;
Santin, Monica ;
Dargatz, David .
VETERINARY PARASITOLOGY, 2010, 170 (3-4) :187-192
[9]   Molecular detection of Enterocytozoon bieneusi and identification of a potentially human-pathogenic genotype in milk [J].
Lee, John Hwa .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (05) :1664-1666
[10]   Prevalence and molecular characteristics of Enterocytozoon bieneusi in cattle in Korea [J].
Lee, John Hwa .
PARASITOLOGY RESEARCH, 2007, 101 (02) :391-396
12