Comprehensive Egg Coat Proteome of the Ascidian Ciona intestinalis Reveals Gamete Recognition Molecules Involved in Self-sterility

被引：39

作者：

Yamada, Lixy ^{[2
]}

Saito, Takako ^{[1
]}

Taniguchi, Hisaaki ^{[2
,3
]}

Sawada, Hitoshi ^{[1
]}

Harada, Yoshito ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Sugashima Marine Biol Lab, Toba 5170004, Japan

[2] Univ Tokushima, Inst Enzyme Res, Div Dis Prote, Tokushima 7708503, Japan

[3] Japan Sci & Technol Agcy JST, Inst Bioinformat Res & Dev BIRD, Chiyoda Ku, Tokyo 1028666, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2009年 / 284卷 / 14期

基金：

日本学术振兴会;

关键词：

ZONA-PELLUCIDA GENES; SELF/NONSELF RECOGNITION; SPERM RECEPTOR; LC-MS/MS; IDENTIFICATION; ALLORECOGNITION; DOMAIN; FERTILIZATION; MECHANISM; EVOLUTION;

D O I：

10.1074/jbc.M809672200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite central roles of egg coat proteins in gamete recognition, their functions and composition are poorly understood. Here, we report that the proteome of the egg coat in the solitary ascidian Ciona intestinalis, called vitelline coat (VC) fraction, contains more than 800 proteins identified by mass spectrometry-based analyses. Over 100 proteins were enriched in the VC fraction compared with the VC-free egg proteome. The most abundant component in the VC was an apolipoprotein-like protein. The VC contained multiple homologs of mammalian zona pellucida (ZP) proteins, the number of which was unexpectedly large and most of which possessed epidermal growth factor-like repeats. Furthermore, the present study revealed that two fibrinogen-like proteins, v-Themis-A and -B, both of which are expressed in the VC, are the molecules responsible for the two self-sterility loci that were identified by our previous genetic study in this species.

引用

页码：9402 / 9410

页数：9

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