Isolation and characterization of microsatellite markers for the causal agent of barley leaf rust, Puccinia hordei

被引：8

作者：

Karaoglu, H. ^{[1
,2
]}

Park, R. F. ^{[1
]}

机构：

[1] Univ Sydney, Plant Breeding Inst, Dept Agr & Environm, Cobbitty, NSW 2570, Australia

[2] Univ Sydney, Plant Breeding Inst, Narellan, NSW 2567, Australia

来源：

AUSTRALASIAN PLANT PATHOLOGY | 2014年 / 43卷 / 01期

关键词：

Puccinia hordei; Microsatellites; Pathotype; Genome; SIMPLE SEQUENCE REPEATS; PATHOGENIC SPECIALIZATION; YIELD; POLYMORPHISM; AUSTRALIA;

D O I：

10.1007/s13313-013-0244-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In this study, we isolated and characterized seventy-six polymorphic microsatellite loci in the barley leaf rust pathogen, Puccinia hordei. The loci were screened with 19 isolates of P. hordei to assess polymorphism. The markers exhibited high variability, with the number of alleles ranging from 3 to 9 with an average of 4.92 alleles per locus. The polymorphic information content (PIC) varied from 0.401 to 0.843 with an average value of 0.624 per locus. These polymorphic microsatellite loci are the first of its type developed for P. hordei and will be extremely useful in studies of population genetics and in monitoring the global movement of this important cereal rust pathogen.

引用

页码：47 / 52

页数：6

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