Modulating effect of D-carvone on 1,2-dimethylhydrazine- induced pre-neoplastic lesions, oxidative stress and biotransforming enzymes, in an experimental model of rat colon carcinogenesis

被引:37
作者
Vinothkumar, R. [1 ]
Sudha, M. [1 ]
Viswanathan, P. [2 ]
Kabalimoorthy, J. [3 ]
Balasubramanian, T. [4 ]
Nalini, N. [1 ]
机构
[1] Annamalai Univ, Fac Sci, Dept Biochem & Biotechnol, Chidambaram 608002, Tamil Nadu, India
[2] Annamalai Univ, Dept Pathol, Raja Muthiah Med Coll, Chidambaram 608002, Tamil Nadu, India
[3] Annamalai Univ, Dept Surg, Raja Muthiah Med Coll, Chidambaram 608002, Tamil Nadu, India
[4] Annamalai Univ, Fac Marine Sci, Ctr Adv Study Marine Biol, Chidambaram 608502, Tamil Nadu, India
关键词
ABERRANT CRYPT FOCI; LIPID-PEROXIDATION; ANTIOXIDANT STATUS; ACID; PURIFICATION; METABOLISM; EFFICACY; CANCER; FIBER; ASSAY;
D O I
10.1111/cpr.12062
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
ObjectivesThe present study has aimed to evaluate chemopreventive potential of d-carvone on oxidative stress markers, biotransforming enzymes, incidence of colonic polyps and aberrant crypt foci (ACF) in 1,2-dimethylhydrazine (DMH)-induced experimental colon carcinogenesis. Materials and MethodsRats were randomly divided into six groups, with group I serving as control. Group II animals received d-carvone every day orally (20mg/kg body weight) for 16weeks; groups III-VI received subcutaneous injections of DMH (20mg/kg body weight) once a week, for the first 4weeks. In addition, groups IV-VI received different doses of d-carvone (5, 10 and 20mg/kg body weight everyday orally) along with DMH injections. ResultsOur results revealed that supplementation with d-carvone significantly reduced incidence of polyps/ACF and ACF multiplicity in DMH-exposed rats compared to DMH-alone-exposed rats. Moreover, our results showed reduced activities of liver and circulatory antioxidants and increased levels of lipid peroxidation by products in DMH-exposed animals, which were significantly reversed on supplementation with d-carvone. In addition, colonic antioxidants and lipid peroxidation were significantly diminished in DMH-exposed rats, which were significantly elevated on supplementation with d-carvone. Furthermore, we also determined activities of biotransforming enzymes, which were found to be altered in DMH-exposed rats, but reversed on d-carvone supplementation. All these observations of changes were supported by histochemical findings. ConclusionOverall, results obtained from this study suggest that d-carvone at 10mg/kg body weight provided optimum protection and could be used as an effective chemopreventive agent against colon carcinogenesis induced by DMH.
引用
收藏
页码:705 / 720
页数:16
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