Real Time In Vivo Measurement of Ascorbate in the Brain Using Carbon Nanotube-Modified Microelectrodes

被引：11

作者：

Ferreira, Nuno R. ^{[1
]}

Santos, Ricardo M. ^{[1
]}

Laranjinha, Joao ^{[1
,2
]}

Barbosa, Rui M. ^{[1
,2
]}

机构：

[1] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal

[2] Univ Coimbra, Fac Pharm, P-3000548 Coimbra, Portugal

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 07期

关键词：

Ascorbate; Carbon fiber microelectrodes; Carbon nanotubes; In vivo electrochemistry; URIC-ACID; FIBER MICROELECTRODES; NITRIC-OXIDE; VOLTAMMETRIC DETECTION; EXTRACELLULAR FLUID; DOPAMINE; RAT; ELECTRODES; GLUTAMATE; NAFION;

D O I：

10.1002/elan.201300053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A carbon fiber microelectrode modified with a composite film of carbon nanotubes and Nafion was developed for in vivo ascorbate (AA) measurements in brain tissue. The modified-microelectrodes exhibit an electrocatalytic activity for AA oxidation by shifting the peak potential negatively to -0.040V, showing a sensitivity of 37pA/mu M, a detection limit of 2.5 mu M, a response time of 0.3s and don't respond to several electroactive compounds found in the brain extracellular space. In the rat hippocampus, the basal concentration of AA was 290 mu M, and glutamate-evoked changes in AA were biphasic comprising fast and slow components.

引用

页码：1757 / 1763

页数：7

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