Cloning and characterization of the full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase

被引：37

作者：

Jung, JW

Shin, WS

Song, J

Lee, ST ^{[1
]}

机构：

[1] Pochon CHA Univ Coll Med, Cell & Gene Therapy Res Inst, Lab Stem Cell Biol, Seoul 135081, South Korea

[2] Yonsei Univ, Coll Sci, Dept Biochem, Natl Res Lab Cellular Biochem, Seoul 120749, South Korea

[3] Yonsei Univ, Prot Network Res Ctr, Seoul 120749, South Korea

来源：

GENE | 2004年 / 328卷

关键词：

embryonic development; mRNA expression; orthologue; tyrosine phosphorylation;

D O I：

10.1016/j.gene.2003.12.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The 3.8-kb full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase was cloned by reverse transcription-PCR of mouse liver mRNA. The mouse PTK7 polypeptide showed 92.6% identity to human PTK7. The mouse Ptk7 gene consists of 20 exons and has exactly the same exon structure as the human PTK7 gene. Mouse PTK7 was shown to be phosphorylated neither by itself nor by other protein tyrosine kinases. In addition, its expression did not affect the phospho-tyrosine level of cellular proteins in COS-1 cells. The mouse Ptk7 mRNA was expressed at high levels in lung and un-pregnant uterus among adult tissues, and in the tail, limbs, somites, gut, and cramofacial regions among embryonic tissues. These data suggest that mouse PTK7, an orthologue of human PTK7, plays multiple roles in embryonic development. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：75 / 84

页数：10

共 39 条

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