Induction of the cell cycle arrest and apoptosis by flavonoids isolated from Korean Citrus aurantium L. in non-small-cell lung cancer cells

被引：49

作者：

Park, Kwang Il ^{[1
,2
]}

Park, Hyeon Soo ^{[1
,2
]}

Nagappan, Arulkumar ^{[1
,2
]}

Hong, Gyeong Eun ^{[1
,2
]}

Lee, Do Hoon ^{[1
,2
]}

Kang, Sang Rim ^{[3
]}

Kim, Jin A. ^{[4
,5
]}

Zhang, Jue

Kim, Eun Hee ^{[6
]}

Lee, Won Sup ^{[7
]}

Shin, Sung Chul ^{[8
,9
]}

Hah, Young Sool ^{[10
]}

Kim, Gon Sup ^{[1
,2
]}

机构：

[1] Gyeongsang Natl Univ, Life Sci Res Inst, Gazwa 660701, Jinju, South Korea

[2] Gyeongsang Natl Univ, Coll Vet Med, Gazwa 660701, Jinju, South Korea

[3] Kyonggi Univ, Sch Nat Sci, Dept Biol Engn, Suwon 443760, South Korea

[4] Gyeongsang Natl Univ, Korea Natl Anim Res Resource Ctr, Gazwa 660701, Jinju, South Korea

[5] Gyeongsang Natl Univ, Korea Natl Anim Bioresource Bank, Gazwa 660701, Jinju, South Korea

[6] Int Univ Korea, Dept Nursing Sci, Jinju 660759, South Korea

[7] Gyeongsang Natl Univ, Gyeongsang Natl Univ Hosp, Sch Med,Dept Internal Med, Inst Hlth Sci,Gyeongnam Reg Canc Ctr, Jinju 660702, South Korea

[8] Gyeongsang Natl Univ, Dept Chem, Jinju 660701, South Korea

[9] Gyeongsang Natl Univ, Life Sci Res Inst, Jinju 660701, South Korea

[10] Gyeongsang Natl Univ Hosp, Clin Res Inst, Jinju 660702, South Korea

来源：

FOOD CHEMISTRY | 2012年 / 135卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Korean Citrus aurantium L; Flavonoid; Human lung cancer cells; Cell cycle arrest; Apoptosis; BCL-2 FAMILY PROTEINS; HUMAN COLON-CANCER; MAMMALIAN-CELLS; PHOSPHORYLATION; KINASE; TRANSITIONS; ACTIVATION; REGULATORS; PATHWAY; DEATH;

D O I：

10.1016/j.foodchem.2012.06.097

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study investigated the anti-proliferative and apoptotic effect of flavonoids isolated from Korean Citrus aurantium L using A549 lung cancer cells. Flavonoids potently inhibited of A549 cells in a dose-dependent manner, whereas flavonoids had a weak inhibitory effect on proliferation of WI-38 cells. Flow cytometry and Western blot analysis showed that flavonoids induced cell cycle arrest at the G2/M check-point by controlling the proteins expression level of cyclin B1, cdc2, cdc25c and p21(WAF1/CIP1). Also, flavonoids induced apoptosis through the regulation of the expression of caspases, cleaved PARP and Bax/Bcl-xL ratio. The activity of caspase-3 on A549 cells increased in a dose-dependent manner. These results clearly indicated that the anti-cancer effect of flavonoids on A549 cells follows multiple cellular pathways through G2/M arrest and the induction of apoptosis. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：2728 / 2735

页数：8

共 32 条

[1] Novel herbal flavonoids promote apoptosis but differentially induce cell cycle arrest in human colon cancer cell [J].