Nozzle design parameters and their effects on rapid sample delivery in flow cytometry

被引:24
作者
Graves, SW
Nolan, JP
Jett, JH
Martin, JC
Sklar, LA
机构
[1] Los Alamos Natl Lab, Biosci Div, Natl Flow Cytometry Resource, Los Alamos, NM 87545 USA
[2] Univ New Mexico, Hlth Sci Ctr, Dept Cytometry, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA
[3] Univ New Mexico, Hlth Sci Ctr, Dept Pathol, Canc Res & Treatment Ctr, Albuquerque, NM 87131 USA
来源
CYTOMETRY | 2002年 / 47卷 / 02期
关键词
flow cytometry; rapid mix; kinetics; high throughput; sheath control;
D O I
10.1002/cyto.10056
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: Rapid kinetic and high throughput flow cytometry are emerging as valuable tools in biotechnology research applications ranging from mechanistic analysis of molecular assemblies to high throughput screening. Many of these new applications have been made possible by improved sample delivery capabilities, focusing increased attention on fluidic issues associated with rapid sample delivery. Methods: Using basic fluidic premises, we derived a model that predicted the effect of nozzle parameters during rapid sample delivery. We tested the model using the rapid mix flow cytometer and modifications were made to the equipment to optimize performance. Results: The model predicted that shorter nozzles with wide exit orifices decrease the delay before initial particle analysis and the fluidic stabilization time. Experimental results confirmed thus prediction and model-based modifications allowed analysis of particles within 55 ms or 600 ms after mixing, with or without electronic gating, respectively. Conclusions: The model along with modifications to commercial equipment will allow rapid mix flow cytometry to analyze reactions in time frames threefold shorter than previously possible. The model allows for nozzle design predictions that should allow for analysis in the millisecond time frame. Furthermore, these findings are general for all rapid delivery applications, including high throughput flow cytometry. Cytometry 47:127-137, 2002. (C) 2002 Wiley-Liss, Inc.
引用
收藏
页码:127 / 137
页数:11
相关论文
共 24 条
[1]  
[Anonymous], 1990, FLOW CYTOM SORTING
[2]  
[Anonymous], FLOW CYTOMETRY INSTR
[3]  
Blankenstein G, 1996, CYTOMETRY, V25, P200, DOI 10.1002/(SICI)1097-0320(19961001)25:2<200::AID-CYTO9>3.0.CO
[4]  
2-K
[5]   TIME WINDOW ANALYSIS AND SORTING [J].
DUNNE, JF .
CYTOMETRY, 1991, 12 (07) :597-601
[6]   A microfabricated fluorescence-activated cell sorter [J].
Fu, AY ;
Spence, C ;
Scherer, A ;
Arnold, FH ;
Quake, SR .
NATURE BIOTECHNOLOGY, 1999, 17 (11) :1109-1111
[7]  
Johnson KA, 1992, ENZYMES, V20, P1, DOI DOI 10.1016/S1874-6047(08)60019-0
[8]   A NEW FLOW CYTOMETRIC TRANSDUCER FOR FAST SAMPLE THROUGHPUT AND TIME RESOLVED KINETIC-STUDIES OF BIOLOGICAL CELLS AND OTHER PARTICLES [J].
KACHEL, V ;
GLOSSNER, E ;
SCHNEIDER, H .
CYTOMETRY, 1982, 3 (03) :202-212
[9]   SAMPLE STATION MODIFICATION PROVIDING ONLINE REAGENT ADDITION AND REDUCED SAMPLE TRANSIT-TIME FOR FLOW CYTOMETERS [J].
KELLEY, KA .
CYTOMETRY, 1989, 10 (06) :796-800
[10]  
Kuckuck FW, 2001, CYTOMETRY, V44, P83, DOI 10.1002/1097-0320(20010501)44:1<83::AID-CYTO1085>3.0.CO