Structural and immunological studies of hetero polysaccharide isolated from the alkaline extract of Tricholoma crassum (Berk.) Sacc

被引：23

作者：

Patra, Pradip ^{[1
]}

Bhanja, Sunil K. ^{[1
]}

Sen, Ipsita K. ^{[1
]}

Nandi, Ashis K. ^{[1
]}

Samanta, Surajit ^{[1
]}

Das, Debsankar ^{[1
]}

Devi, K. Sanjana P. ^{[2
]}

Maiti, Tapas K. ^{[2
]}

Acharya, Krishnendu ^{[3
]}

Islam, Syed S. ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India

[2] IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India

[3] Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, Kolkata 700019, W Bengal, India

来源：

CARBOHYDRATE RESEARCH | 2012年 / 362卷

关键词：

Tricholoma crassum (Berk.) Sacc; Hetero polysaccharide; Structure; NMR Spectroscopy; Immunological study; EDIBLE MUSHROOM; OLIGOSACCHARIDES; CONFIGURATION; ANTITUMOR; FUNGI;

D O I：

10.1016/j.carres.2012.09.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An immunoenhancing water-soluble hetero polysaccharide was isolated from an alkaline extract of the fruit bodies of an ectomycorrhizal edible mushroom, Tricholoma crassum (Berk.) Sacc. The structure of the molecule was investigated using acid hydrolysis, methylation analysis; periodate oxidation study, and NMR spectroscopy techniques (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as: [GRAPHICS] This polysaccharide exhibited splenocyte, thymocyte as well as macrophage activations. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1 / 7

页数：7

共 31 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].

AGRAWAL, PK .

PHYTOCHEMISTRY, 1992, 31 (10) :3307-3330

[2] Isolation and characterization of the immunostimulating β-glucans of an edible mushroom Termitomyces robustus var. [J].

Bhanja, Sunil K. ;

Nandan, Chanchal K. ;

Mandal, Soumitra ;

Bhunia, Bibhas ;

Maiti, Tapas K. ;

Mondal, Soumitra ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2012, 357 :83-89

[3]

Chang ST., 1978, BIOL CULTIVATION EDI

[4] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[5] A (1→6)-β-glucan from a somatic hybrid of Pleurotus florida and Volvariella volvacea: isolation, characterization, and study of immunoenhancing properties [J].

Das, Debsankar ;

Mondal, Subhas ;

Roy, Sadhan K. ;

Maiti, Debabrata ;

Bhunia, Bibhas ;

Maiti, Tapas K. ;

Sikdar, Samir R. ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2010, 345 (07) :974-978

[6] Structural analysis of the exopolysaccharide produced by Pediococcus damnosus 2.6 [J].

DuenasChasco, HT ;

RodriguezCarvajal, MA ;

Mateo, PT ;

FrancoRodriguez, G ;

Espartero, JL ;

IrastorzaIribas, A ;

GilSerrano, AM .

CARBOHYDRATE RESEARCH, 1997, 303 (04) :453-458

[7] POLYSACCHARIDES IN PHARMACY - CURRENT APPLICATIONS AND FUTURE CONCEPTS [J].

FRANZ, G .

PLANTA MEDICA, 1989, (06) :493-497

[8] Selective tumoricidal effect of soluble proteoglucan extracted from the basidiomycete, Agaricus blazei Murill, mediated via natural killer cell activation and apoptosis [J].

Fujimiya, Y ;

Suzuki, Y ;

Oshiman, K ;

Kobori, H ;

Moriguchi, K ;

Nakashima, H ;

Matumoto, Y ;

Takahara, S ;

Ebina, T ;

Katakura, R .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 1998, 46 (03) :147-159

[9] DETERMINATION OF D AND L CONFIGURATION OF NEUTRAL MONOSACCHARIDES BY HIGH-RESOLUTION CAPILLARY GLC [J].

GERWIG, GJ ;

KAMERLING, JP ;

VLIEGENTHART, JFG .

CARBOHYDRATE RESEARCH, 1978, 62 (02) :349-357

[10]

GOLDSTEIN I. J., 1965, METHOD CARBOHYD CHEM, V5, P361

← 1 2 3 4 →