Antioxidant effect of p-coumaric acid on interleukin 1-β and tumor necrosis factor-α in rats with renal ischemic reperfusion

Background and aims: Renal ischemia-reperfusion occurs in some clinical conditions such as kidney surgery that can leads to acute renal failure. The aim of this study was to investigate the effect of p-coumaric acid (CA) on ischemia reperfusion (I/R) injury. Methods: Thirty rats were randomly divided into five groups; control, CA (100 mg/kg), I/R, propylene glycol (10%) + I/R and CA + I/R, (n = 6 each). CA and propylene glycol were administered orally for 2 weeks. Then, the rats were subjected to bilateral renal ischemia for 45 min and followed by reperfusion for 24 h. All rats were killed and kidney function tests, tissue malondialdehyde and activity of antioxidant enzymes were determined. Histopathological evaluations were also performed. In addition, renal expression of the tumor necrosis factor-alpha and interleukin-1 beta were determined using enzyme-linked immunosorbent assay and immunohistochemistry. Results: CA significantly improved the Cr and BUN levels in CA + I/R group compared to I/R group (p < 0.005 and p < 0.001, respectively). Reduction of tissue superoxide dismutase, glutathione peroxidase and catalase, were significantly improved by CA (p < 0.01, p < 0.01 and p < 0.05). Treatment with CA also resulted in significant reduction in tissue MDA (p < 0.05), TNF-alpha (p < 0.001) and interleukin-1 beta expression (p < 0.001) that were increased by renal I/R. Also, the rats treated with CA had nearly normal structure of the kidney. Conclusions: The present findings suggest that, CA protects the kidneys against I/R injury via its antioxidant and anti-inflammatory effects. (C) 2019 Sociedad Espanola de Nefrologia. Published by Elsevier Espana, S.L.U.