Evaluation of the In Vivo Antioxidant Activity of Mucuna pruriens DC. var. utilis by Using Caenorhabditis elegans

被引：1
作者：
Ibe, Sachie [1 ]
Fujii, Yoshiharu [2 ]
Otobe, Kazunori [3 ,4 ]
机构：
[1] Ohyamatofu Co Ltd, Isehara, Kanagawa 2591147, Japan
[2] Tokyo Univ Agr & Technol, Fuchu, Tokyo 1838509, Japan
[3] Natl Agr & Food Res Org, Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan
[4] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058666, Japan
来源：
FOOD SCIENCE AND TECHNOLOGY RESEARCH | 2012年 / 18卷 / 02期
关键词：
Mucuna pruriens; antioxidant; L-DOPA; Caenorhabditis elegans; GERMPLASM SEED MATERIALS; L-DOPA; ANTINUTRITIONAL FACTORS; OXIDATIVE STRESS; TRIBAL PULSE; EXTRACT; MECHANISM; L-3,4-DIHYDROXYPHENYLALANINE; FERMENTATION; NATTO;
D O I：
10.3136/fstr.18.227
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Mucuna pruriens (MP) is a legume with seeds that contain substantial amounts of 3, 4-dihydroxy-L-phenylalanine (L-DOPA; 3% to 7% dry weight). We examined the in vivo antioxidant activity of distilled-water extracts of steamed or fermented MP seeds in Caenorhabditis elegans to evaluate the potential use of MP as a functional food. Paraquat and thermal stress generate reactive oxygen species in C. elegans. Worms treated with MP extracts were exposed to acute stress with paraquat or lethal heat shock. The survival rate 3 days after the treatment was used to indicate the antioxidant activity of the MP extracts. Distilled-water extracts of steamed or fermented MP had significantly higher (P < 0.01) antioxidant activity than in the control (nematode standard media) in vivo. L-DOPA showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity but did not contribute to the antioxidant activity of MP in vivo.
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页码：227 / 233
页数：7
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