Prognostic value of cell-cycle regulators and cellular biomarkers in laryngeal squamous cell carcinoma

Objective: Combining information derived from cellular biomarkers as telomerase and genes encoding cyclin dependent kinase inhibitors (p 16(INK4a) and p15(INK4b)) is needed to facilitate the stratification of individual patients within the conventional clinicopathologic parameters. Design and methods: One hundred forty laryngeal squamous cell carcinoma (LSCC) tissue specimens were investigated for telomerase activity and the deletions of p16(INK4a) and P15(INK4b) genes. Results: Of the 140 tissues assayed, 71% demonstrated high telomerase activity, 68.6% and 80% showed deletions in p16(INK4a) and p15(INK4b) genes, respectively. Significant difference was observed between telomerase activity, p16(INK4a) and p15(INK4b) deletions among each other and with advanced stage, poorly differentiated grades, high proliferation and DNA aneuploidy. The markers and the aforementioned clinicopathological factors were correlated with progression in univariate analysis; and associated with survival in multivariate analysis. Conclusion: Progression of LSCC is accompanied by a parallel increase in telomerase activity and deletions in cell-cycle regulators (p16(INK4a) p15(INK4b)) which may assist in prognostication and better classification of patients for treatment. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.