Effects of levofloxacin exposure on sequencing batch reactor (SBR) behavior and microbial community changes

被引：33

作者：

Hao, Liting ^{[1
,2
]}

Okano, Kunihiro ^{[3
]}

Zhang, Chi ^{[1
]}

Zhang, Zhenya ^{[1
]}

Lei, Zhongfang ^{[1
]}

Feng, Chuanping ^{[2
]}

Utsumi, Motoo ^{[1
]}

Ihara, Ikko ^{[4
]}

Maseda, Hideaki ^{[5
]}

Shimizu, Kazuya ^{[1
]}

机构：

[1] Univ Tsukuba, Grad Sch Life & Environm Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058572, Japan

[2] China Univ Geosci Beijing, Sch Water Resources & Environm, MOE Key Lab Groundwater Circulat & Environm Evolu, Beijing 100083, Peoples R China

[3] Akita Prefectural Univ, Fac Bioresource Sci, Dept Biol Environm, 241-438 Kaidobata Nishi, Akita, Akita 0100195, Japan

[4] Kobe Univ, Fac Agr, Nada Ku, 1-1 Rokkodai Cho, Kobe, Hyogo 6578501, Japan

[5] Natl Inst Adv Ind Sci & Technol, 1-8-31 Midorigaoka, Ikeda, Osaka 5638577, Japan

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2019年 / 672卷

关键词：

Levofloxacin (LVX); Antibiotics contaminated wastewater; Nitrogen removal; Microbial community; Sequencing batch reactor (SBR); AMMONIA-OXIDIZING BACTERIA; WASTE-WATER; DNA GYRASE; CIPROFLOXACIN; ANTIBIOTICS; DIVERSITY; OXIDATION; SEDIMENT; NITROGEN;

D O I：

10.1016/j.scitotenv.2019.03.272

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The adaptation mechanisms of bacterial community for nitrogen removal performance exposed to fluctuated levels of levofloxacin (LVX) during wastewater treatment in SBRs were investigated. Although LVX is completely synthetic, the results of minimum inhibitory concentration (MIC, 32 mg-LVX/L) and minimum bactericidal concentration (MBC, 512 mg-LVX/L) of the sampled sludge showed that the LVX resistance/tolerance for bacterial growth has already existed in the actual wastewater treatment plants (WWTPs). The key bacteria, i.e. Nitrosomonas sp. (ammonia-oxidizing bacteria), Nitrospira sp. (nitrite-oxidizing bacteria) and Thauera sp. (the predominant denitrifiers), decreased with LVX exposure, and the recovery of biological process in the reactor was disturbed due to LVX exposure. However, after stopping exposure their population was quickly increased and thus the performance was recovered. The results of the non-metric multidimensional scaling and microbial community by sequencing showed the LVX concentration was a crucial factor to the change of bacterial communities and controlled the quantitative evolution of the communities in our systems. This effect was more pronounced as the LVX concentration was higher. The results suggested the removal of residual antibiotics to accomplish under no effect concentration before biological treatment is important to suppress emerging and increasing of the antibiotic resistant bacteria in WWTPs. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：227 / 238

页数：12

共 33 条

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