Agreement between allergen-specific IgE assays and ensuing immunotherapy recommendations from four commercial laboratories in the USA

被引:17
作者
Plant, Jon D. [1 ]
Neradelik, Moni B. [2 ]
Polissar, Nayak L. [2 ]
Fadok, Valerie A. [3 ]
Scott, Brian A. [4 ]
机构
[1] SkinVet Clin, Lake Oswego, OR 97035 USA
[2] Mt Whisper Light Stat, Natori, Miyagi 98112, Japan
[3] North Houston Vet Specialists, Spring, TX 77388 USA
[4] South Texas Vet Specialists, San Antonio, TX 78258 USA
关键词
CANINE ATOPIC-DERMATITIS; ACVD TASK-FORCE; COCKTAIL-BASED ELISA; CROSS-REACTIVITY; DOGS; TESTS; REPRODUCIBILITY; PERFORMANCE; GUIDELINES; DIAGNOSIS;
D O I
10.1111/vde.12104
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
BackgroundCanine allergen-specific IgE assays in the USA are not subjected to an independent laboratory reliability monitoring programme. Hypothesis/ObjectivesThe aim of this study was to evaluate the agreement of diagnostic results and treatment recommendations of four serum IgE assays commercially available in the USA. MethodsReplicate serum samples from 10 atopic dogs were submitted to each of four laboratories for allergen-specific IgE assays (ACTT((R)), VARL Liquid Gold, ALLERCEPT((R)) and Greer((R)) Aller-g-complete((R))). The interlaboratory agreement of standard, regional panels and ensuing treatment recommendations were analysed with the kappa statistic () to account for agreement that might occur merely by chance. Six comparisons of pairs of laboratories and overall agreement among laboratories were analysed for ungrouped allergens (as tested) and also with allergens grouped according to reported cross-reactivity and taxonomy. ResultsThe overall chance-corrected agreement of the positive/negative test results for ungrouped and grouped allergens was slight (=0.14 and 0.13, respectively). Subset analysis of the laboratory pair with the highest level of diagnostic agreement (=0.36) found slight agreement (=0.13) for ungrouped plants and fungi, but substantial agreement ( = 0.71) for ungrouped mites. The overall agreement of the treatment recommendations was slight (=0.11). Altogether, 85.1% of ungrouped allergen treatment recommendations were unique to one laboratory or another. Conclusions and clinical importanceOur study indicated that the choice of IgE assay may have a major influence on the positive/negative results and ensuing treatment recommendations.
引用
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页码:15 / +
页数:10
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