Aging-associated exacerbation in fatty degeneration and infiltration after rotator cuff tear

被引:69
作者
Gumucio, Jonathan P. [1 ,2 ]
Korn, Michael A. [1 ,2 ]
Saripalli, Anjali L. [1 ]
Flood, Michael D. [1 ]
Phan, Anthony C. [1 ]
Roche, Stuart M. [1 ]
Lynch, Evan B. [1 ,2 ]
Claflin, Dennis R. [3 ,4 ]
Bedi, Asheesh [1 ]
Mendias, Christopher L. [1 ,2 ]
机构
[1] Univ Michigan, Sch Med, Dept Orthopaed Surg, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Sch Med, Dept Mol & Integrat Physiol, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Sch Med, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Sch Med, Dept Surg, Sect Plast Surg, Ann Arbor, MI 48109 USA
基金
美国国家卫生研究院;
关键词
Fatty degeneration; rotator cuff; sarcopenia; atrophy; lipid droplets; macrophages; SKELETAL-MUSCLE; INSULIN-RESISTANCE; LIPID-METABOLISM; TENDON TEAR; RAT MODEL; OLD RATS; ATROPHY; FORCE; DIFFERENTIATION; REGENERATION;
D O I
10.1016/j.jse.2013.04.011
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Background: Rotator cuff tears are one of the most common musculoskeletal complaints and a substantial source of morbidity in elderly patients. Chronic cuff tears are associated with muscle atrophy and an infiltration of fat to the area, a condition known as "fatty degeneration." To improve the treatment of cuff tears in elderly patients, a greater understanding of the changes in the contractile properties of muscle fibers and the molecular regulation of fatty degeneration is essential. Methods: Using a full-thickness, massive supraspinatus and infraspinatus tear model in elderly rats, we measured fiber contractility and determined changes in fiber type distribution that develop 30 days after tear. We also measured the expression of messenger RNA and micro-RNA transcripts involved in muscle atrophy, lipid accumulation, and matrix synthesis. We hypothesized that a decrease in specific force of muscle fibers, an accumulation of type IIb fibers, and an upregulation in atrophic, fibrogenic, and inflammatory gene expression would occur in torn cuff muscles. Results: Thirty days after the tear, we observed a reduction in muscle fiber force and an induction of RNA molecules that regulate atrophy, fibrosis, lipid accumulation, inflammation, and macrophage recruitment. A marked accumulation of advanced glycation end products and a significant accretion of macrophages in areas of fat accumulation were observed. Conclusions: The extent of degenerative changes in old rats was greater than that observed in adults. In addition, we identified that the ectopic fat accumulation that occurs in chronic cuff tears does not occur by activation of canonical intramyocellular lipid storage and synthesis pathways. Level of evidence: Basic Science Study, Molecular and Cell Biology, Animal Model. (C) 2014 Journal of Shoulder and Elbow Surgery Board of Trustees.
引用
收藏
页码:99 / 108
页数:10
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