Evaluation of cytotoxic effects of dichloromethane extract of Guduchi (Tinospora cordifolia Miers ex Hook F & THOMS) on cultured HeLa cells

被引：31

作者：

Jagetia, Ganesh Chandra ^{[1
]}

Rao, Shaival Kamalaksha ^{[1
]}

机构：

[1] Kasturba Med Coll & Hosp, Dept Radiobiol, Manipal 576104, India

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2006年 / 3卷 / 02期

关键词：

cytotoxicity; glutathione-S-transferase; Guduchi; HeLa; lactate dehydrogenase; lipid peroxidation;

D O I：

10.1093/ecam/nel011

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Extracts of Tinospora cordifolia (TCE) have been shown to possess anti-tumor properties, but the mechanism of the anti-tumor function of TCE is poorly understood. This investigation elucidates the possible mechanism underlying the cytotoxic effects of dichlormethane extracts of TCE, after selecting optimal duration and concentration for treatment. HeLa cells were exposed to various concentrations of TCE, which has resulted in a concentration-dependent decline in the clonogenicity, glutathione-S-transferase (GST) activity and a concentration-dependent increase in lipid peroxidation (TBARS) with a peak at 4 h and lactate dehydrogenase (LDH) release with a peak at 2 h. Our results suggest that the cytotoxic effect of TCE may be due to lipid peroxidation and release of LDH and decline in GST.

引用

页码：267 / 272

页数：6

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