Multi-Layer Micro-Nanofluidic Device for Isolation and Capture of Extracellular Vesicles Derived From Liposarcoma Cell Conditioned Media

被引：0
作者：
Sundaram, Prashanth Mohana [1 ]
Casadei, Lucia [2 ]
Lopez, Gonzalo [2 ]
Braggio, Danielle [2 ]
Balakirsky, Gita [2 ]
Pollock, Raphael [2 ]
Prakash, Shaurya [1 ,2 ]
机构：
[1] Ohio State Univ, Dept Mech & Aerosp Engn, Columbus, OH 43210 USA
[2] Ohio State Univ, Comprehens Canc Ctr, Columbus, OH 43210 USA
来源：
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS | 2020年 / 29卷 / 05期
基金：
美国国家卫生研究院;
关键词：
Silicon; Biomembranes; Extracellular; Plasmas; Cancer; DNA; Scanning electron microscopy; Extracellular vesicles; exosomes; microfluidics; nanofluidics; liposarcoma; cancer; EXOSOMES; MDM2; BENIGN; TISSUE; LIPOMA;
D O I：
10.1109/JMEMS.2020.3006786
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We report on isolation, capture, and subsequent elution for analysis of extracellular vesicles derived from human liposarcoma cell conditioned media, using a multi-layer micro-nanofluidic device operated with tangential flow separation. Our device integrates size-based separation followed by immunoaffinity-based capture of extracellular vesicles in the same device. For liposarcomas, this is the first report on isolating, capturing, and then eluting the extracellular vesicles using a micro-nanofluidic device. The results show a significantly higher yield of the eluted extracellular vesicles (similar to 84%) compared to the current methods of ultracentrifugation (similar to 6%) and ExoQuick-based separations (similar to 16%). [2020-0155]
引用
收藏
页码：776 / 782
页数：7
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