Crystallization and preliminary crystallographic studies of putative threonyl-tRNA synthetases from Aeropyrum pernix and Sulfolobus tokodaii

被引：2

作者：

Shimizu, Satoru ^{[1
]}

Juan, Ella Czarina Magat ^{[1
]}

Miyashita, Yu-ichiro ^{[1
]}

Sato, Yoshiteru ^{[1
]}

Hoque, Md Mominul ^{[1
]}

Suzuki, Kaoru ^{[2
]}

Yogiashi, Masataka ^{[2
]}

Tsunoda, Masaru ^{[3
]}

Dock-Bregeon, Anne-Catherine ^{[4
]}

Moras, Dino ^{[4
]}

Sekiguchi, Takeshi ^{[2
]}

Takenaka, Akio ^{[1
,3
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[2] Iwaki Meisei Univ, Coll Sci & Engn, Fukushima 9708551, Japan

[3] Iwaki Meisei Univ, Fac Pharm, Fukushima 9708551, Japan

[4] Inst Genet & Biol Mol & Cellulaire, Dept Biol & Genom Struct, F-67404 Illkirch Graffenstaden, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷

关键词：

D O I：

10.1107/S1744309108026924

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Threonyl-tRNA synthetase (ThrRS) plays an essential role in protein synthesis by catalyzing the aminoacylation of tRNA(Thr) and editing misacylation. ThrRS generally contains an N-terminal editing domain, a catalytic domain and an anticodon-binding domain. The sequences of the editing domain in ThrRSs from archaea differ from those in bacteria and eukaryotes. Furthermore, several creanarchaea including Aeropyrum pernix K1 and Sulfolobus tokodaii strain 7 contain two genes encoding either the catalytic or the editing domain of ThrRS. To reveal the structural basis for this evolutionary divergence, the two types of ThrRS from the crenarchaea A. pernix and S. tokodaii have been overexpressed in Eschericha coli, purified and crystallized by the hanging-drop vapour-diffusion method. Diffraction data were collected and the structure of a selenomethionine-labelled A. pernix type-1 ThrRS crystal has been solved using the MAD method.

引用

页码：903 / 910

页数：8

共 28 条

[1] Methods used in the structure determination of bovine mitochondrial F-1 ATPase [J].

Abrahams, JP ;

Leslie, AGW .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1996, 52 :30-42

[2] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[3] A domain for editing by an archaebacterial tRNA synthetase [J].

Beebe, K ;

Merriman, E ;

Pouplana, LR ;

Schimmel, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (16) :5958-5963

[4] FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES [J].

BRUNGER, AT .

NATURE, 1992, 355 (6359) :472-475

[5] Glu-tRNA(Gln) amidotransferase: A novel heterotrimeric enzyme required for correct decoding of glutamine codons during translation [J].

Curnow, AW ;

Hong, KW ;

Yuan, R ;

Kim, SI ;

Martins, O ;

Winkler, W ;

Henkin, TM ;

Soll, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (22) :11819-11826

[6] A 2ND CLASS OF SYNTHETASE STRUCTURE REVEALED BY X-RAY-ANALYSIS OF ESCHERICHIA-COLI SERYL-TRANSFER RNA-SYNTHETASE AT 2.5-A [J].

CUSACK, S ;

BERTHETCOLOMINAS, C ;

HARTLEIN, M ;

NASSAR, N ;

LEBERMAN, R .

NATURE, 1990, 347 (6290) :249-255

[7] Achieving error-free translation: The mechanism of proofreading of threonyl-tRNA synthetase at atomic resolution [J].

Dock-Bregeon, AC ;

Rees, B ;

Torres-Larios, A ;

Bey, G ;

Caillet, J ;

Moras, D .

MOLECULAR CELL, 2004, 16 (03) :375-386

[8] PARTITION OF TRANSFER-RNA SYNTHETASES INTO 2 CLASSES BASED ON MUTUALLY EXCLUSIVE SETS OF SEQUENCE MOTIFS [J].

ERIANI, G ;

DELARUE, M ;

POCH, O ;

GANGLOFF, J ;

MORAS, D .

NATURE, 1990, 347 (6289) :203-206

[9] ENZYME HYPER-SPECIFICITY - REJECTION OF THREONINE BY VALYL-TRANSFER-RNA SYNTHETASE BY MIS-ACYLATION AND HYDROLYTIC EDITING [J].

FERSHT, AR ;

KAETHNER, MM .

BIOCHEMISTRY, 1976, 15 (15) :3342-3346

[10] Post-transfer editing mechanism of a D-aminoacyl-tRNA deacylase-like domain in threonyl-tRNA synthetase from archaea [J].

Hussain, Tanweer ;

Kruparani, Shobha P. ;

Pal, Biswajit ;

Dock-Bregeon, Anne-Catherine ;

Dwivedi, Shweta ;

Shekar, Megala R. ;

Sureshbabu, Kotini ;

Sankaranarayanan, Rajan .

EMBO JOURNAL, 2006, 25 (17) :4152-4162

← 1 2 3 →