Surface Display of a Redox Enzyme and its Site-Specific Wiring to Gold Electrodes

被引:41
作者
Amir, Liron [1 ,2 ,3 ,4 ]
Carnally, Stewart A. [1 ,2 ,4 ]
Rayo, Josep [3 ,4 ]
Rosenne, Shaked [3 ,4 ]
Yerushalmi, Sarit Melamed [3 ,4 ]
Schlesinger, Orr [1 ,2 ,4 ]
Meijler, Michael M. [3 ,4 ]
Alfonta, Lital [1 ,2 ,4 ]
机构
[1] Ben Gurion Univ Negev, Avram & Stella Goldstein Goren Dept Biotechnol En, IL-84105 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Ilse Katz Inst Nanoscale Sci & Technol, IL-84105 Beer Sheva, Israel
[3] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel
[4] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel
基金
欧洲研究理事会; 以色列科学基金会;
关键词
MICROBIAL FUEL-CELLS; PROTEINS; CHEMISTRY; BIOSENSOR; GLUCOSE;
D O I
10.1021/ja310556n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The generation of a current through interaction between bacteria and electrodes has been explored by various methods. We demonstrate the attachment of living bacteria through a surface displayed redox enzyme, alcohol dehydrogenase II. The unnatural amino acid para-azido-L-phenylalanine was incorporated into a specific site of the displayed enzyme, facilitating electron transfer between the enzyme and an electrode. In order to attach the bacteria carrying the surface displayed enzyme to a surface, a linker containing an alkyne and a thiol moiety on opposite ends was synthesized and attached to the dehydrogenase site specifically through a copper(I)-catalyzed azide-alkyne cydoaddition reaction. Using this approach we were able to covalently link bacteria to gold-coated surfaces and to gold nanoparticles, while maintaining viability and catalytic activity. We show the performance of a biofuel cell using these modified bacteria at the anode, which resulted in site-specific dependent fuel cell performance for at least a week. This is the first example of site-specific attachment of a true living biohybrid to inorganic material.
引用
收藏
页码:70 / 73
页数:4
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