Performance limits of monolithic and packed capillary columns in high-performance liquid chromatography and capillary electrochromatography

被引：63
作者：
Eeltink, S
Desmet, G
Vivó-Truyols, G
Rozing, GP
Schoenmakers, PJ
Kok, WT
机构：
[1] Univ Amsterdam, Polymer Anal Grp, Van Hoff Inst Mol Sci, NL-1018 WV Amsterdam, Netherlands
[2] Free Univ Brussels, Dept Chem Engn, B-1050 Brussels, Belgium
[3] Agilent Technol, Pharmaceut Solut, Life Sci & Chem Anal, D-76337 Waldbronn, Germany
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1104卷 / 1-2期
关键词：
column characterization; performance limits; microparticulate columns; silica monoliths; metbacrylate monoliths; efficiency;
D O I：
10.1016/j.chroma.2005.11.112
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is proposed for the comprehensive characterization and comparison of columns in the high-performance liquid chromatographic (HPLC) and capillary electrochromatographic (CEC) modes. Using this approach, column parameters such as the number of plates, the eddy-diffusion and mass-transfer contributions to peak broadening, the permeability, and the analysis time are incorporated in a single graph and a comparison in terms of efficiency and speed is obtained. The chromatographic performance of silica-based and polymer-based monolithic capillary columns is discussed and a comparison is made with the performance of packed columns. Also, the potential of ultra-high-pressure liquid chromatography is discussed in this context. In the HPLC mode, the best results were obtained with silica monoliths; in the CEC mode, the low-density methacrylate-ester-based monoliths showed the best performance. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：256 / 262
页数：7
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