Impact of age-associated increase in 2′-O-methylation of miRNAs on aging and neurodegeneration in Drosophila

被引:45
作者
Abe, Masashi [1 ]
Naqvi, Ammar [2 ]
Hendriks, Gert-Jan [1 ]
Feltzin, Virzhiniya [1 ]
Zhu, Yongqing [1 ]
Grigoriev, Andrey [2 ]
Bonini, Nancy M. [1 ]
机构
[1] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
[2] Rutgers State Univ, Dept Biol, Ctr Computat & Integrat Biol, Camden, NJ 08102 USA
基金
美国国家科学基金会;
关键词
miRNA; 2 '-O-methylation; miRNA sorting; aging; neurodegeneration; PIWI-INTERACTING RNAS; SMALL SILENCING RNAS; CAENORHABDITIS-ELEGANS; LIFE-SPAN; MICRORNA EXPRESSION; ENDOGENOUS SIRNAS; DISTINCT ROLES; SOMATIC-CELLS; C-ELEGANS; PATHWAY;
D O I
10.1101/gad.226654.113
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
MicroRNAs (miRNAs) are 20- to similar to 24-nucleotide (nt) small RNAs that impact a variety of biological processes, from development to age-associated events. To study the role of miRNAs in aging, studies have profiled the levels of miRNAs with time. However, evidence suggests that miRNAs show heterogeneity in length and sequence in different biological contexts. Here, by examining the expression pattern of miRNAs by Northern blot analysis, we found that Drosophila miRNAs show distinct isoform pattern changes with age. Surprisingly, an increase of some miRNAs reflects increased 2'-O-methylation of select isoforms. Small RNA deep sequencing revealed a global increase of miRNAs loaded into Ago2, but not into Ago1, with age. Our data suggest increased loading of miRNAs into Ago2, but not Ago1, with age, indicating a mechanism for differential loading of miRNAs with age between Ago1 and Ago2. Mutations in Hen1 and Ago2, which lack 2'-O-methylation of miRNAs, result in accelerated neurodegeneration and shorter life span, suggesting a potential impact of the age-associated increase of 2'-O-methylation of small RNAs on age-associated processes. Our study highlights that miRNA 2'-O-methylation at the 39 end is modulated by differential partitioning of miRNAs between Ago1 and Ago2 with age and that this process, along with other functions of Ago2, might impact age-associated events in Drosophila.
引用
收藏
页码:44 / 57
页数:14
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