Genetic diversity of the honeybee Apis cerana in Yunnan, China, based on mitochondrial DNA

被引:10
作者
Yin, L. [1 ]
Ji, T. [1 ]
机构
[1] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Peoples R China
关键词
Apis cerana; Genetic diversity; Genetic differentiation; mtDNA; INTEGRATED SOFTWARE; BIOGEOGRAPHY; HYMENOPTERA; APIDAE;
D O I
10.4238/2013.June.20.1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DNA sequence diversity in the tRNA(leu)-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNA(leu)-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 were new. The mean diversity of haplotypes was 0.752 +/- 0.030 (0.378-0.698), and nucleotide diversity was 0.01073 +/- 0.00087 (0.00412-0.01123). A neighbor-joining tree was constructed based on 73 sequences of noncoding intergenic regions in the mtDNA of A. cerana from China and other Asian countries; all haplotypes found in China fell into the mainland Asian group. This result does not support the hypothesis that A. cerana indica occurs in southern Yunnan, which was concluded in a recent report based on morphological variation.
引用
收藏
页码:2002 / 2009
页数:8
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