Generation of Transgenic Rats through Induced Pluripotent Stem Cells

被引：13

作者：

Jiang, Ming-Gui ^{[1
,2
,3
]}

Li, Tianda ^{[1
,3
]}

Feng, Chunjing ^{[1
,3
,4
]}

Fu, Rui ^{[1
,3
]}

Yuan, Yan ^{[1
,5
]}

Zhou, Quan ^{[1
,5
]}

Li, Xin ^{[1
,6
]}

Wan, Haifeng ^{[1
,3
]}

Wang, Liu ^{[1
,3
]}

Li, Wei ^{[1
,3
]}

Xiao, Yamei ^{[2
]}

Zhao, Xiao-Yang ^{[1
,3
]}

Zhou, Qi ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China

[2] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China

[3] Chinese Acad Sci, Inst Zool, Key Lab Translat Stem Cell Res, Beijing 100101, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[5] Nanjing Med Univ, Dept Histol & Embryol, State Key Lab Reprod Med, Nanjing 210000, Jiangsu, Peoples R China

[6] Northeast Agr Univ China, Coll Life Sci, Harbin 150030, Peoples R China

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2013年 / 288卷 / 38期

关键词：

D O I：

10.1074/jbc.M113.492900

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rat is an important animal model for human disease research. Using inhibitors of glycogen synthase kinase 3 and MAPK signaling pathways, rat embryonic stem cells and rat induced pluripotent stem cells (riPSCs) have been derived. However, unlike rat embryonic stem cells, germ line competent riPSCs have only been derived from Wistar rats at low efficiency. Here, we found that an optimized induction medium containing knock-out serum replacement and vitamin C improved the rate and efficiency of riPSCs generation from Dark Agouti rat fibroblasts and Sertoli cells. riPSCs maintained an undifferentiated status for > 30 passages and could differentiate into various cells types including germ cells when injected into rat blastocysts. Moreover, transgenic riPSCs could be generated through the PiggyBac transposon, which could be used to generate transgenic rats through germ line transmission. riPSCs can be used as a novel tool in genetic and genomic studies of the rat.

引用

页码：27150 / 27158

页数：9

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