Microwave-assisted extraction, HPLC analysis, and inhibitory effects on carbonic anhydrase I, II, VA, and VII isoforms of 14 blueberry Italian cultivars

被引：48
作者：
Mollica, Adriano [1 ]
Locatelli, Marcello [1 ]
Macedonio, Giorgia [1 ]
Carradori, Simone [1 ]
Sobolev, Anatoly P. [2 ]
De Salvador, Roberto F. [3 ]
Monti, Simona M. [4 ]
Buonanno, Martina [4 ,5 ]
Zengin, Gokhan [6 ]
Angeli, Andrea [7 ]
Supuran, Claudiu T. [7 ,8 ]
机构：
[1] GD Annunzio Univ Chieti Pescara, Dept Pharm, Chieti, Italy
[2] CNR, Inst Chem Methodol, Magnet Resonance Lab Annalaura Segre, Rome, Italy
[3] Ctr Ric Frutticoltura, CRA Consiglio Ric Sperimentaz Agr, Rome, Italy
[4] Ist Biostrutture & Bioimmagini CNR, Naples, Italy
[5] Seconda Univ Napoli, Dipartimento Sci & Tecnol Ambientali, Biol & Farmaceut, Caserta, Italy
[6] Selcuk Univ, Fac Sci, Dept Biol, Konya, Turkey
[7] Univ Florence, Lab Chim Bioinorgan, Florence, Italy
[8] Univ Florence, Dept Neurofarba, Sect Pharmaceut & Nutriceut Sci, Florence, Italy
来源：
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY | 2016年 / 31卷
关键词：
Blueberry; carbonic anhydrase inhibitors; cultivar; HPLC-PDA; microwave-assisted extraction; OXYPRENYLATED ANTHRAQUINONES; ANTIOXIDANT; CHROMATOGRAPHY; ANTHOCYANINS; PROFILES; FRUITS; MILL; BARK;
D O I：
10.1080/14756366.2016.1214951
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The multi-component fingerprint and the biological evaluation of plant-derived material are indispensable for the pharmaceutical field, in food quality control procedures, and in all plant based products. We investigated the quantitative content of biologically active compounds (anthocyanins and chlorogenic acid) of microwave-assisted blueberry extracts from 14 different Italian cultivars, using validated high-performance liquid chromatography-photodiode array detector (HPLC-PDA) method and routinely instrument configuration. The carbonic anhydrase (CA, EC 4.2.1.1) inhibition profiles against several pharmacologically relevant CA isoforms of blueberry extracts and some bioactive compounds were also investigated. The various cultivars showed a highly variable content in anthocyanins and chlorogenic acid, and their CA inhibitory effects were also highly variable. Overall these data prove that antioxidant natural products found in blueberries may be useful for designing pharmacological agents in which various CAs are involved, e.g., antiobesity, antitumor, or anticonvulsants agents.
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页码：1 / 6
页数：6
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