Enhanced Secretion of Biologically Active Murine Interleukin-12 by Lactococcus lactis

被引：20
|
作者：
Fernandez, Antonio [1 ]
Horn, Nikki [1 ]
Wegmann, Udo [1 ]
Nicoletti, Claudio [1 ]
Gasson, Michael J. [1 ]
Narbad, Arjan [1 ]
机构：
[1] BBSRC Inst Food Res, Norwich NR4 7UA, Norfolk, England
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 03期
关键词：
CONTROLLED EXPRESSION; NISIN; STRAINS; SIGNAL; CYTOKINE; ANTIGEN; GENES; MICE;
D O I：
10.1128/AEM.01728-08
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The novel signal peptide SLPmod was used for the secretion of murine interleukin-12 (mIL-12) by Lactococcus lactis. A >4-fold increase in secretion was observed when SLPmod was used instead of the Usp45-derived secretion signal. Oral delivery of this cytokine using the autoinducible host L. lactis FI5876 utilizing SLPmod resulted in a significant increase in mIL-12 plasma levels in mice.
引用
收藏
页码：869 / 871
页数：3
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