Enhanced Secretion of Biologically Active Murine Interleukin-12 by Lactococcus lactis

被引：20

作者：

Fernandez, Antonio ^{[1
]}

Horn, Nikki ^{[1
]}

Wegmann, Udo ^{[1
]}

Nicoletti, Claudio ^{[1
]}

Gasson, Michael J. ^{[1
]}

Narbad, Arjan ^{[1
]}

机构：

[1] BBSRC Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 03期

关键词：

CONTROLLED EXPRESSION; NISIN; STRAINS; SIGNAL; CYTOKINE; ANTIGEN; GENES; MICE;

D O I：

10.1128/AEM.01728-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The novel signal peptide SLPmod was used for the secretion of murine interleukin-12 (mIL-12) by Lactococcus lactis. A >4-fold increase in secretion was observed when SLPmod was used instead of the Usp45-derived secretion signal. Oral delivery of this cytokine using the autoinducible host L. lactis FI5876 utilizing SLPmod resulted in a significant increase in mIL-12 plasma levels in mice.

引用

页码：869 / 871

页数：3

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