Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy

被引：35
|
作者：
Bag, Nirmalya [1 ,2 ,3 ]
Ali, Ashraf [4 ]
Chauhan, Virander Singh [4 ]
Wohland, Thorsten [1 ,2 ,3 ]
Mishra, Aseem [4 ]
机构：
[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117557, Singapore
[2] Natl Univ Singapore, Dept Chem, Singapore 117557, Singapore
[3] Natl Univ Singapore, NUS Ctr Bioimaging Sci CBIS, Singapore 117557, Singapore
[4] Int Ctr Genet Engn & Biotechnol, Malaria Res Grp, New Delhi 110067, India
来源：
CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 80期
关键词：
ISLET AMYLOID POLYPEPTIDE; DIFFUSION; ORGANIZATION;
D O I：
10.1039/c3cc44880k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Monomeric hIAPP significantly destabilizes both model and live cell membranes by increasing membrane fluidity. This interaction with membranes happens via carpet formation followed by lipid extraction in a concentration dependent manner and thus we propose that hIAPP aggregation prior to membrane interaction may not be necessary for its cytotoxicity.
引用
收藏
页码：9155 / 9157
页数：3
相关论文
共 50 条
  • [31] Site-specific interaction of thrombin and inhibitors observed by fluorescence correlation spectroscopy
    Klingler, J
    Friedrich, T
    BIOPHYSICAL JOURNAL, 1997, 73 (04) : 2195 - 2200
  • [32] Application of fluorescence correlation spectroscopy for velocity imaging in microfluidic devices
    Kuricheti, KK
    Buschmann, V
    Weston, KD
    APPLIED SPECTROSCOPY, 2004, 58 (10) : 1180 - 1186
  • [33] Velocity imaging in microfluidic devices using fluorescence correlation spectroscopy
    Kuricheti, KK
    Buschmann, V
    Brister, P
    Weston, KD
    MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS II, 2004, 5345 : 194 - 205
  • [34] ASBESTOS-MEDIATED MEMBRANE UPTAKE OF BENZO[A]PYRENE OBSERVED BY FLUORESCENCE SPECTROSCOPY
    LAKOWICZ, JR
    HYLDEN, JL
    NATURE, 1978, 275 (5679) : 446 - 448
  • [35] Plasma Membrane Diffusion Modes of FcεRI Receptor for Immunoglobin E Measured with Imaging Fluorescence Correlation Spectroscopy
    Bag, Nirmalya
    Holowka, David
    Baird, Barbara
    BIOPHYSICAL JOURNAL, 2017, 112 (03) : 28A - 28A
  • [36] Fluorescence correlation spectroscopy
    Webb, WW
    PHOTON CORRELATION AND SCATTERING, TECHNICAL DIGEST, 2000, 47 : 4 - 6
  • [37] Fluorescence correlation spectroscopy
    Ries, Jonas
    Schwille, Petra
    BIOESSAYS, 2012, 34 (05) : 361 - 368
  • [38] Fluorescence correlation spectroscopy
    Müller, JD
    Chen, Y
    Gratton, E
    BIOPHOTONICS, PT B, 2003, 361 : 69 - 92
  • [39] FLUORESCENCE CORRELATION SPECTROSCOPY
    WEBB, WW
    BULLETIN OF THE AMERICAN PHYSICAL SOCIETY, 1974, 19 (03): : 197 - 197
  • [40] Fluorescence Correlation Spectroscopy
    Beranova, Lenka
    Humpolickova, Jana
    Hof, Martin
    CHEMICKE LISTY, 2009, 103 (02): : 125 - 129
12345