Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy

被引：35

作者：

Bag, Nirmalya ^{[1
,2
,3
]}

Ali, Ashraf ^{[4
]}

Chauhan, Virander Singh ^{[4
]}

Wohland, Thorsten ^{[1
,2
,3
]}

Mishra, Aseem ^{[4
]}

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117557, Singapore

[2] Natl Univ Singapore, Dept Chem, Singapore 117557, Singapore

[3] Natl Univ Singapore, NUS Ctr Bioimaging Sci CBIS, Singapore 117557, Singapore

[4] Int Ctr Genet Engn & Biotechnol, Malaria Res Grp, New Delhi 110067, India

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 80期

关键词：

ISLET AMYLOID POLYPEPTIDE; DIFFUSION; ORGANIZATION;

D O I：

10.1039/c3cc44880k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Monomeric hIAPP significantly destabilizes both model and live cell membranes by increasing membrane fluidity. This interaction with membranes happens via carpet formation followed by lipid extraction in a concentration dependent manner and thus we propose that hIAPP aggregation prior to membrane interaction may not be necessary for its cytotoxicity.

引用

页码：9155 / 9157

页数：3

共 50 条

[1] Bayesian Analysis of Imaging FCS Investigates the Interaction of Monomeric HIAPP with Live Cell Membrane
Bag, Nirmalya
Guo, Syuan-Ming
Mishra, Aseem
Bathe, Mark
Wohland, Thorsten
BIOPHYSICAL JOURNAL, 2014, 106 (02) : 295A - 295A
[2] Imaging Fluorescence Correlation Spectroscopy Characterizes Membrane Dynamics and Organization
Bag, Nirmalya
BIOPHYSICAL JOURNAL, 2012, 102 (03) : 303A - 303A
[3] Applications of imaging fluorescence correlation spectroscopy
Singh, Anand P.
Wohland, Thorsten
CURRENT OPINION IN CHEMICAL BIOLOGY, 2014, 20 : 29 - 35
[4] Membrane Anchor Dependent Colocalization in Cellular Membranes Observed by Fluorescence Cross-Correlation Spectroscopy
Triffo, Sara B.
Huang, Hector H.
Smith, Adam W.
Groves, Jay T.
BIOPHYSICAL JOURNAL, 2010, 98 (03) : 305A - 305A
[5] Excitation saturation in fluorescence correlation spectroscopy and fluorescence imaging
Berland, K
BIOPHYSICAL JOURNAL, 2004, 86 (01) : 334A - 334A
[6] Fluorescence correlation spectroscopy and fluorescence lifetime imaging microscopy
Breusegem, Sophia Y.
Levi, Moshe
Barry, Nicholas P.
NEPHRON EXPERIMENTAL NEPHROLOGY, 2006, 103 (02): : E41 - E49
[7] Bayesian Total Internal Reflection Fluorescence Correlation Spectroscopy Reveals hIAPP-Induced Plasma Membrane Domain Organization in Live Cells
Guo, Syuan-Ming
Bag, Nirmalya
Mishra, Aseem
Wohland, Thorsten
Bathe, Mark
BIOPHYSICAL JOURNAL, 2014, 106 (01) : 190 - 200
[8] Fluorescence correlation spectroscopy in membrane structure elucidation
Chiantia, Salvatore
Ries, Jonas
Schwille, Petra
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2009, 1788 (01): : 225 - 233
[9] Fluorescence correlation spectroscopy close to a fluctuating membrane
Fradin, C
Abu-Arish, A
Granek, R
Elbaum, M
BIOPHYSICAL JOURNAL, 2003, 84 (03) : 2005 - 2020
[10] Membrane anchor dependent protein colocalization in cellular membranes observed by fluorescence cross-correlation spectroscopy
Triffo, Sara B.
Huang, Hector H.
Smith, Adam W.
Groves, Jay T.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2010, 239

← 1 2 3 4 5 →