Optimization of Extraction Parameters by Using Response Surface Methodology, Purification, and Identification of Anthocyanin Pigments in Melastoma malabathricum Fruit

被引:44
作者
Anuar, Nordiyanah [1 ]
Adnan, Ahmad Faris Mohd [1 ,2 ]
Saat, Naziz [1 ]
Aziz, Norkasmani [1 ,2 ]
Taha, Rosna Mat [1 ,2 ]
机构
[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia
[2] Univ Malaya, Ctr Ion, Dept Phys, Kuala Lumpur 50603, Malaysia
关键词
D O I
10.1155/2013/810547
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell. Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that can be recovered from Melastoma malabathricum fruit. Factors such as extraction temperature, extraction time, and solid to liquid ratio were identified to be significantly affecting anthocyanin extraction efficiency. By using three-level three-factor Box-Behnken design, the optimized conditions for anthocyanin extraction by acidified methanol (R-2 = 0.972) were temperature of 60 degrees C, time of 86.82 min, and 0.5:35 (g/mL) solid to liquid ratio while the optimum extraction conditions by acidified ethanol (R-2 = 0.954) were temperature of 60 degrees C, time of 120 min, and 0.5:23.06 (g/mL) solid to liquid ratio. The crude anthocyanin extract was further purified by using Amberlite XAD-7 and Sephadex LH-20 column chromatography. Identification of anthocyanins revealed the presence of cyanidin dihexoside, cyanidin hexoside, and delphinidin hexoside as the main anthocyanins in M. malabathricum fruit.
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页数:10
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