An investigation of antibody immobilization methods employing organosilanes on planar ZnO surfaces for biosensor applications

被引:67
作者
Corso, Christopher D. [2 ]
Dickherber, Anthony [1 ]
Hunt, William D. [1 ]
机构
[1] Georgia Inst Technol, Dept Elect Engn, Atlanta, GA 30332 USA
[2] Georgia Inst Technol, Dept Biomed Engn, Atlanta, GA 30332 USA
基金
美国国家科学基金会;
关键词
Organosilane; Antibody immobilization; ZnO; Acoustic sensor; Crosslinker;
D O I
10.1016/j.bios.2008.07.011
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
One critical aspect for the development of label-free immunosensors is the employment of highly uniform and repeatable antibody immobilization techniques. In this study, we investigated the use of two different silane molecules (3-glycidyloxypropyl)trimethoxysilane (GPS), and (3-mercaptopropyl)trimethoxysilane (MTS) for the immobilization of fluorescently labeled IgG antibodies on planar ZnO surfaces. The chemical modification of the surfaces was investigated using water contact angle measurements, AFM, and fluorescence microscopy. The results of the water contact angle measurements indicate increased surface hydrophobicity after treatment with CPS and MTS as compared to the control. Surface modification was further verified through AFM measurements which demonstrate an increased surface roughness and particle height after treatment with antibodies. The results of the fluorescence studies indicate that the immobilization protocol employing MTS produced 21% higher fluorescence on average with greater uniformity than the GPS-based protocol, which indicates a higher overall density in antibody coverage on the surface of the ZnO. Acoustic sensor tests were employed to confirm the functionality of sensors treated with the MTS protocol. The results indicate that the immobilization protocol imparts sensitivity and specificity to the ZnO-based devices. (C) 2008 Published by Elsevier B.V.
引用
收藏
页码:805 / 811
页数:7
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