Comparison of MAS-PCR and GenoType MTBDR assay for the detection of rifampicin-resistant Mycobacterium tuberculosis

被引：0

作者：

Tho, D. Q. ^{[1
]}

Ha, D. T. M. ^{[1
,2
]}

Duy, P. M. ^{[1
]}

Lan, N. T. N. ^{[2
]}

Hoa, D. V. ^{[2
]}

Chau, N. V. V.

Farrar, J. ^{[1
,3
]}

Caws, M. ^{[1
,3
]}

机构：

[1] Univ Oxford, Hosp Trop Dis, Clin Res Unit, Ho Chi Minh City, Vietnam

[2] Pham Ngoc Thach Hosp TB & Lung Dis, Ho Chi Minh City, Vietnam

[3] Churchill Hosp, Ctr Clin Vaccinol & Trop Med, Oxford OX3 7LJ, England

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2008年 / 12卷 / 11期

关键词：

tuberculosis; rifampicin; resistance; PCR; MTBDR;

D O I：

暂无

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

SETTING: Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases, the tertiary referral hospital for tuberculosis (TB) in Southern Vietnam. OBJECTIVE: To develop and evaluate a simple, rapid and accurate multiplex allele specific polymerase chain reaction (MAS-PCR) test to detect refampicin (RMP) resistance point mutations at codons 516, 526 or 531 in the rpoB gene of Mycobacterium tuberculosis. DESIGN: The novel MAS-PCR was compared with the commercial M. tuberculosis Drug Resistance (MTBDR) test in 104 RMP-resistant and 50 RMP-susceptible routine isolates, defined by conventional 1% phenotypic susceptibility testing. RESULTS: The sensitivity of the MAS-PCR and MTBDR tests was respectively 83.7% (95%CI 75.1-90.2) and 93.3% (95%Cl 86.6-97.3). Both tests were 100% specific. The negative predictive value was 74.6% (95%CI 65.3-83.1) for the MAS-PCR and 87.7% (95%CI 80.0-93.6) for the MTBDR test. CONCLUSION: The MTBDR test, although more sensitive is currently prohibitively expensive in resourcc-poor, high-burden settings. The MAS-PCR described here presents a less laborious economic alternative. A susceptible result returned by either test cannot be used to exclude

引用

页码：1306 / 1312

页数：7