Detection of brain tumors using fluorescence diffuse optical tomography and nanoparticles as contrast agents

被引：21

作者：

Fortin, Pierre-Yves ^{[1
,2
]}

Genevois, Coralie ^{[1
,2
]}

Koenig, Anne ^{[3
]}

Heinrich, Emilie ^{[3
]}

Texier, Isabelle ^{[3
]}

Couillaud, Franck ^{[1
,4
]}

机构：

[1] Univ Bordeaux 2, CNRS, UMR 5231, Univ Bordeaux Segalen,Lab IMF, F-33076 Bordeaux, France

[2] Univ Bordeaux 2, CNRS, Inst BioImagerie, Univ Bordeaux Segalen,UMS 3428, F-33076 Bordeaux, France

[3] LETI Minatec, CEA, Grenoble, France

[4] Univ Bordeaux 2, CNRS, Lab RMSB, Univ Bordeaux Segalen,UMR 5536, F-33076 Bordeaux, France

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 12期

关键词：

tomography; bioluminescence; fluorescence; magnetic resonance imaging; cancer imaging; nanoparticles; IN-VIVO; MEDIA;

D O I：

10.1117/1.JBO.17.12.126004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Near-infrared fluorescence-enhanced diffuse optical tomography (fDOT) is used to localize tumors in mice using fluorescent nanoparticles as a blood pool contrast agent. The infrared dye DiR is loaded in the lipid core of nontargeted nanoparticles (DiR-lipidots) and injected systemically via the tail vein in mice bearing U87 tumors. Distribution and time-course of DiR-lipidots are followed using in vivo fluorescence reflectance imaging and reveal enhanced fluorescent signal within the subcutaneous tumors up to seven days due to the enhanced permeability and retention effect. Tumor growth into the brain is followed using bioluminescent imaging, and tumor localization is further determined by magnetic resonance imaging. The fDOT provides three-dimensional fluorescent maps that allow for consistent localization for both subcutaneous and brain tumors. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.12.126004]

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页数：6

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