Design and Structure of an Engineered Disulfide-Stabilized Influenza Virus Hemagglutinin Trimer

被引：34

作者：

Lee, Peter S. ^{[1
,2
]}

Zhu, Xueyong ^{[1
]}

Yu, Wenli ^{[1
]}

Wilson, Ian A. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 14期

关键词：

ANTIBODY RECOGNITION; GLYCOPROTEIN; IMMUNOGEN; EPITOPES; BINDING; ORIGIN; DOMAIN; MICE;

D O I：

10.1128/JVI.00808-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer, termed HA3-SS, by introducing cysteine residues into the HA stem to covalently bridge the three protomers. HA3-SS has increased thermostability compared to wild-type HA, and binding of head-and stem-targeted antibodies (Abs) is preserved; only minor structural changes are found in the vicinity of the additional disulfide. This platform has been applied to H1 and H3 HAs and provides prospects for design of intact, stabilized influenza virus HA immunogens.

引用

页码：7417 / 7420

页数：4

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