Altered Glutamate Receptor Ionotropic Delta Subunit 2 Expression in Stau2-Deficient Cerebellar Purkinje Cells in the Adult Brain

被引：13

作者：

Pernice, Helena F. ^{[1
]}

Schieweck, Rico ^{[1
]}

Jafari, Mehrnoosh ^{[2
,3
]}

Straub, Tobias ^{[4
]}

Bilban, Martin ^{[5
]}

Kiebler, Michael A. ^{[1
]}

Popper, Bastian ^{[1
,6
]}

机构：

[1] Ludwig Maximilians Univ Munchen, Fac Med, Dept Cell Biol & Anat, Biomed Ctr BMC, D-82152 Martinsried, Germany

[2] Ludwig Maximilians Univ Munchen, Inst Clin Neuroimmunol, Univ Hosp, D-81377 Munich, Germany

[3] Ludwig Maximilians Univ Munchen, Biomed Ctr, D-81377 Munich, Germany

[4] Ludwig Maximilians Univ Munchen, Fac Med, Core Facil Bioinformat, Biomed Ctr BMC, D-82152 Martinsried, Germany

[5] Med Univ Vienna, Dept Lab Med & Core Facil Genom, A-1090 Vienna, Austria

[6] Ludwig Maximilians Univ Munchen, Fac Med, Core Facil Anim Models, Biomed Ctr BMC, D-82152 Martinsried, Germany

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 07期

关键词：

staufen2; synaptogenesis; cerebellum; purkinje cells; GluD2; LONG-TERM DEPRESSION; MOTOR COORDINATION; SYNAPSE FORMATION; CBLN1; GLUR-DELTA-2; PLASTICITY; STAUFEN2; PROTEINS; NEUREXIN; ROLES;

D O I：

10.3390/ijms20071797

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Staufen2 (Stau2) is an RNA-binding protein that is involved in dendritic spine morphogenesis and function. Several studies have recently investigated the role of Stau2 in the regulation of its neuronal target mRNAs, with particular focus on the hippocampus. Here, we provide evidence for Stau2 expression and function in cerebellar Purkinje cells. We show that Stau2 downregulation (Stau2(GT)) led to an increase of glutamate receptor ionotropic delta subunit 2 (GluD2) in Purkinje cells when animals performed physical activity by voluntary wheel running compared with the age-matched wildtype (WT) mice (C57Bl/6J). Furthermore, Stau2(GT) mice showed lower performance in motor coordination assays but enhanced motor learning abilities than did WT mice, concomitantly with an increase in dendritic GluD2 expression. Together, our results suggest the novel role of Stau2 in Purkinje cell synaptogenesis in the mouse cerebellum.

引用

页数：12

共 39 条

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