Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases

被引：25
|
作者：
Biesaga, M [1 ]
Orska, J [1 ]
Fiertek, D [1 ]
Izdebski, J [1 ]
Trojanowicz, M [1 ]
机构：
[1] Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 364卷 / 1-2期
关键词：
D O I：
10.1007/s002160051316
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The retention of Selected dipeptides and tripeptides containing tyrosine was examined; As stationary phase an aminopropylated silica gel loaded with covalently linked tetraphenylporphyrin was used. The effect of metalization of porphyrin with Cu(II) and Zn(II) on retention was investigated. The observed separation is based on a mixed mechanism involving pi-pi and hydrophobic interactions as well as complex formation between immobilized metal ions and peptides. A satisfactory separation was demonstrated for C-peptide and bovine insulin. The possibility of:separation of various insulins was also investigated.
引用
收藏
页码：160 / 164
页数：5
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