Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases

被引：25
|
作者：
Biesaga, M [1 ]
Orska, J [1 ]
Fiertek, D [1 ]
Izdebski, J [1 ]
Trojanowicz, M [1 ]
机构：
[1] Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland
来源：
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1999年 / 364卷 / 1-2期
关键词：
D O I：
10.1007/s002160051316
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The retention of Selected dipeptides and tripeptides containing tyrosine was examined; As stationary phase an aminopropylated silica gel loaded with covalently linked tetraphenylporphyrin was used. The effect of metalization of porphyrin with Cu(II) and Zn(II) on retention was investigated. The observed separation is based on a mixed mechanism involving pi-pi and hydrophobic interactions as well as complex formation between immobilized metal ions and peptides. A satisfactory separation was demonstrated for C-peptide and bovine insulin. The possibility of:separation of various insulins was also investigated.
引用
收藏
页码：160 / 164
页数：5
相关论文
共 50 条
  • [31] Nanoengineered immobilized metal affinity chromatography stationary phases for high resolution protein and peptide separations
    McCarthy, P
    Chattopadhyay, M
    Tracy, M
    Shimmin, D
    Millhauser, G
    Rao, S
    Lee, S
    Sun, GQ
    Avdalovic, N
    Pohl, C
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 : U190 - U190
  • [32] CHELATING PEPTIDE-IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY - A NEW CONCEPT IN AFFINITY-CHROMATOGRAPHY FOR RECOMBINANT PROTEINS
    SMITH, MC
    FURMAN, TC
    INGOLIA, TD
    PIDGEON, C
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1988, 263 (15) : 7211 - 7215
  • [33] CHELATING PEPTIDE-IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY - A NEW CONCEPT IN AFFINITY-CHROMATOGRAPHY OF RECOMBINANT PROTEINS
    SMITH, MC
    FURMAN, TC
    PIDGEON, C
    BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1987, 368 (07): : 775 - 776
  • [34] Histidines in affinity tags and surface clusters for immobilized metal-ion affinity chromatography of trimeric tumor necrosis factor α
    Gaberc-Porekar, V
    Menart, V
    Jevsevar, S
    Vidensek, A
    Stalc, A
    JOURNAL OF CHROMATOGRAPHY A, 1999, 852 (01) : 117 - 128
  • [35] A novel hierarchical nanozeolite composite as sorbent for protein separation in immobilized metal-ion affinity chromatography
    Xu, F
    Wang, YJ
    Wang, XD
    Zhang, YH
    Tang, Y
    Yang, PY
    ADVANCED MATERIALS, 2003, 15 (20) : 1751 - +
  • [36] NEW DEVELOPMENTS IN IMMOBILIZED METAL-ION CHROMATOGRAPHY, IMAC
    PORATH, J
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1992, 203 : 86 - ANYL
  • [37] Purification and folding of recombinant bovine oxoglutarate/malate carrier by immobilized metal-ion affinity chromatography
    Smith, VR
    Walker, JE
    PROTEIN EXPRESSION AND PURIFICATION, 2003, 29 (02) : 209 - 216
  • [38] PURIFICATION OF MILK WHEY ALPHA-LACTALBUMIN BY IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY
    BOYER, RF
    JOURNAL OF CHEMICAL EDUCATION, 1991, 68 (05) : 430 - 432
  • [39] IMMOBILIZED METAL-ION AFFINITY ELECTROPHORESIS - A PRELIMINARY-REPORT
    GOUBRANBOTROS, H
    VIJAYALAKSHMI, MA
    ELECTROPHORESIS, 1991, 12 (12) : 1028 - 1032
  • [40] COPPER(II) IMINODIACETIC ACID CHELATING RESIN AS A STATIONARY PHASE IN THE IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY OF SOME AROMATIC-AMINES
    LIU, Y
    YU, SL
    JOURNAL OF CHROMATOGRAPHY, 1990, 515 : 169 - 173
12345