Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples

被引：23

作者：

Sathyavathi, R. ^{[1
]}

Dingari, Narahara Chari ^{[2
]}

Barman, Ishan ^{[2
]}

Prasad, P. S. R. ^{[3
]}

Prabhakar, Subhashini ^{[4
]}

Rao, D. Narayana ^{[1
]}

Dasari, Ramachandra R. ^{[2
]}

Undamatla, Jayanthi ^{[5
]}

机构：

[1] Univ Hyderabad, Sch Phys, Hyderabad 500134, Andhra Pradesh, India

[2] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA

[3] Natl Geophys Res Inst, Council Sci & Ind Res, Hyderabad 500007, Andhra Pradesh, India

[4] Apollo Hosp, Hyderabad, Andhra Pradesh, India

[5] Apollo Hosp Educ & Res Fdn, Hyderabad, Andhra Pradesh, India

来源：

JOURNAL OF BIOPHOTONICS | 2013年 / 6卷 / 08期

关键词：

tuberculous meningitis (TBM); cerebrospinal fluid (CSF); Raman spectroscopy; silicates; IDENTIFICATION; MYCOBACTERIUM; BACTERIAL; SYSTEM; CELLS;

D O I：

10.1002/jbio.201200110

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this letter, we propose a novel method for diagnosis of tuberculous meningitis using Raman spectroscopy. The silicate Raman signature obtained from Mycobacterium tuberculosis positive cases enables specific and sensitive detection of tuberculous meningitis from acquired cerebrospinal fluid samples. The association of silicates with the tuberculosis mycobacterium is discussed. We envision that this new method will facilitate rapid diagnosis of tuberculous meningitis without application of exogenous reagents or dyes and can be aptly used as a complementary screening tool to the existing gold standard methods.

引用

页码：567 / 572

页数：6

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