Single Cell Volume Measurement Utilizing the Fluorescence Exclusion Method (FXm)

被引：3
|
作者：
Rochman, Nash [1 ]
Yao, Kai [2 ]
Gonzalez, Nicolas Perez [1 ]
Wirtz, Denis [1 ,3 ]
Sun, Sean [1 ,2 ,3 ]
机构：
[1] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA
[2] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD 21218 USA
[3] Johns Hopkins Univ, Phys Sci Oncol Ctr PSOC, Baltimore, MD 21218 USA
来源：
BIO-PROTOCOL | 2020年 / 10卷 / 12期
基金：
美国国家卫生研究院;
关键词：
Single cell; Cell mechanics; Epifluorescence microscopy; Microfluidics; Image analysis; Neural networks; MICROSCOPY;
D O I：
10.21769/BioProtoc.3652
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The measurement of single cell size remains an obstacle towards a deeper understanding of cell growth control, tissue homeostasis, organogenesis, and a wide range of pathologies. Recent advances have placed a spotlight on the importance of cell volume in the regulation of fundamental cell signaling pathways including those known to orchestrate progression through the cell cycle. Here we provide our protocol for the Fluorescence Exclusion Method (FXm); references to the development of FXm; and a brief outlook on future advances in image analysis which may expand the range of problems studied utilizing FXm as well as lower the barrier to entry for groups interested in adding cell volume measurements into their experimental repertoire.
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页数：11
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