Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy

被引：33

作者：

Axpe, Eneko ^{[1
]}

Lopez-Euba, Tamara ^{[2
]}

Castellanos-Rubio, Ainara ^{[2
,3
]}

Merida, David ^{[4
]}

Angel Garcia, Jose ^{[4
]}

Plaza-Izurieta, Leticia ^{[2
,3
]}

Fernandez-Jimenez, Nora ^{[2
,3
]}

Plazaola, Fernando ^{[1
]}

Ramon Bilbao, Jose ^{[2
,3
]}

机构：

[1] Univ Basque Country UPV EHU, Dept Elect & Elect, Leioa, Basque Country, Spain

[2] BioCruces Res Inst, Immunogenet Res Lab, Baracaldo, Basque Country, Spain

[3] Univ Basque Country UPV EHU, Dept Genet Phys Anthropol & Anim Physiol, Leioa, Basque Country, Spain

[4] Univ Basque Country UPV EHU, Dept Appl Phys 2, Leioa, Basque Country, Spain

来源：

PLOS ONE | 2014年 / 9卷 / 01期

关键词：

SKIN;

D O I：

10.1371/journal.pone.0083838

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Positron annihilation lifetime spectroscopy (PALS) provides a direct measurement of the free volume void sizes in polymers and biological systems. This free volume is critical in explaining and understanding physical and mechanical properties of polymers. Moreover, PALS has been recently proposed as a potential tool in detecting cancer at early stages, probing the differences in the subnanometer scale free volume voids between cancerous/healthy skin samples of the same patient. Despite several investigations on free volume in complex cancerous tissues, no positron annihilation studies of living cancer cell cultures have been reported. We demonstrate that PALS can be applied to the study in human living 3D cell cultures. The technique is also capable to detect atomic scale changes in the size of the free volume voids due to the biological responses to TGF-beta. PALS may be developed to characterize the effect of different culture conditions in the free volume voids of cells grown in vitro.

引用

页数：5

共 15 条

[1]

[Anonymous], 1988, Positron annihilation studies of fluids

[2] Applications of positron annihilation spectroscopy to life science [J].