Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid

被引：7

作者：

Kaihatsu, Kunihiro ^{[1
]}

Sawada, Shinjiro ^{[1
]}

Nakamura, Shota ^{[2
]}

Nakaya, Takaaki ^{[3
]}

Yasunaga, Teruo ^{[2
]}

Kato, Nobuo ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka, Japan

[2] Osaka Univ, Microbial Dis Res Inst, Osaka, Japan

[3] Kyoto Prefectural Univ Med, Dept Infect Dis, Kyoto, Japan

来源：

PLOS ONE | 2013年 / 8卷 / 05期

关键词：

DUPLEX DNA; PNA; TRANSCRIPTION; RECOGNITION; INHIBITION; BINDING;

D O I：

10.1371/journal.pone.0064017

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.

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页数：5

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