Self-Assembly of Antibodies by Chemical Induction

被引：23

作者：

Li, Qing ^{[2
]}

Hapka, David ^{[3
]}

Chen, Him ^{[4
]}

Vallera, Daniel A. ^{[4
]}

Wagner, Carston R. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Med Chem, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Coll Pharm, Minneapolis, MN 55455 USA

[4] Univ Minnesota, Dept Therapeut Radiol, Minneapolis, MN 55455 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 52期

基金：

美国国家卫生研究院;

关键词：

antibiotics; antibodies; diabodies; macrocyclization; self-assembly;

D O I：

10.1002/anie.200803507

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Getting together and breaking up: Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis-MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti-CD3 single-chain variable region (scFv) self-assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：10179 / 10182

页数：4

共 20 条

[1] Novel tetravalent and bispecific IgG-like antibody molecules combining single-chain diabodies with the immunoglobulin γl Fc or CH3 region [J].