BODIPY-Based Ratiometric Fluorescent Sensor for Highly Selective Detection of Glutathione over Cysteine and Homocysteine

被引：868

作者：

Niu, Li-Ya ^{[1
,2
]}

Guan, Ying-Shi ^{[1
,2
]}

Chen, Yu-Zhe ^{[1
]}

Wu, Li-Zhu ^{[1
]}

Tung, Chen-Ho ^{[1
]}

Yang, Qing-Zheng ^{[1
]}

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2012年 / 134卷 / 46期

基金：

中国国家自然科学基金;

关键词：

LIVING CELLS; CELLULAR GLUTATHIONE; HYDROGEN-SULFIDE; PROBE; DYES; THIOLS; FUNCTIONALIZATION; NAPHTHALIMIDE; RHODAMINE; REDUCTASE;

D O I：

10.1021/ja309079f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a ratiometric fluorescent sensor based on monochlorinated BODIPY for highly selective detection of glutathione (GSH) over cysteine (Cys)/homocysteine (Hcy). The chlorine of the monochlorinated BODIPY can be rapidly replaced by thiolates of biothiols through thiol halogen nucleophilic substitution. The amino groups of Cys/Hcy but not GSH further replace the thiolate to form amino-substituted BODIPY. The significantly different photophysical properties of sulfur- and amino-substituted BODIPY enable the discrimination of GSH over Cys and Hcy. The sensor was applied for detection of GSH in living cells.

引用

页码：18928 / 18931

页数：4

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