Effect of fluoroquinolone concentration on selection of resistant mutants of Mycobacterium bovis BCG and Staphylococcus aureus

被引：224

作者：

Dong, YZ

Zhao, XL

Domagala, J

Drlica, K

机构：

[1] Publ Hlth Res Inst City New York Inc, New York, NY 10016 USA

[2] Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Ann Arbor, MI 48105 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 07期

关键词：

D O I：

10.1128/AAC.43.7.1756

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

When Mycobacterium bovis BCG and Staphylococcus aureus were plated on agar containing increasing concentrations of fluoroquinolone, colony numbers exhibited a sharp drop, followed by a plateau and a second sharp drop. The plateau region correlated,vith the presence of first-step resistant mutants. Mutants were not recovered at concentrations above those required for the second sharp drop, thereby defining a mutant prevention concentration (MPC). A C-8-methoxy group lowered the MPC for an N-1-cyclopropyl fluoroquinolone.

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收藏

页码：1756 / 1758

页数：3

相关论文

共 15 条

[1] Differential behaviors of Staphylococcus aureus and Escherichia coli type II DNA topoisomerases [J].

Blanche, F ;

Cameron, B ;

Bernard, FX ;

Maton, L ;

Manse, B ;

Ferrero, L ;

Ratet, N ;

Lecoq, C ;

Goniot, A ;

Bisch, D ;

Crouzet, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (12) :2714-2720

[2] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[3] Fluoroquinolone action against mycobacteria: Effects of C-8 substituents on growth, survival, and resistance [J].

Dong, YZ ;

Xu, C ;

Zhao, XL ;

Domagala, J ;

Drlica, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (11) :2978-2984

[4] DNA gyrase, topoisomerase IV, and the 4-quinolones [J].

Drlica, K ;

Zhao, XL .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1997, 61 (03) :377-+

[5] CLONING AND PRIMARY STRUCTURE OF STAPHYLOCOCCUS-AUREUS DNA TOPOISOMERASE-IV - A PRIMARY TARGET OF FLUOROQUINOLONES [J].

FERRERO, L ;

CAMERON, B ;

MANSE, B ;

LAGNEAUX, D ;

CROUZET, J ;

FAMECHON, A ;

BLANCHE, F .

MOLECULAR MICROBIOLOGY, 1994, 13 (04) :641-653

[6] ANALYSIS OF GYRA AND GRLA MUTATIONS IN STEPWISE-SELECTED CIPROFLOXACIN-RESISTANT MUTANTS OF STAPHYLOCOCCUS-AUREUS [J].

FERRERO, L ;

CAMERON, B ;

CROUZET, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (07) :1554-1558

[7]

JACOBS WR, 1991, METHOD ENZYMOL, V204, P537

[8] N-1-tert-butyl-substituted quinolones: In vitro anti Mycobacterium avium activities and structure-activity relationship studies [J].

Klopman, G ;

Fercu, D ;

Renau, TE ;

Jacobs, MR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (11) :2637-2643

[9]

Nakamura S, 1997, J INFECT CHEMOTHER, V3, P128, DOI 10.1007/BF02491502

[10] Quinolone resistance mutations in topoisomerase IV: Relationship to the flqA locus and genetic evidence that topoisomerase IV is the primary target and DNA gyrase is the secondary target of fluoroquinolones in Staphylococcus aureus [J].

Ng, EY ;

Trucksis, M ;

Hooper, DC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (08) :1881-1888