Metabolomics analysis based on UHPLC-Q-TOF-MS/MS reveals effects of genistein on reducing mycotoxin citrinin production by Monascus aurantiacus Li AS3.4384

被引：20

作者：

He, Shanshan ^{[1
,2
]}

Liu, Xin ^{[1
,2
]}

Wang, Yanling ^{[1
,2
]}

Xie, Jianhua ^{[1
]}

Gao, Heng ^{[1
,2
]}

Li, Xiujiang ^{[3
]}

Huang, Zhibing ^{[1
,2
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China

[2] Nanchang Univ, Sino German Joint Res Inst, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China

[3] Nanchang Univ, Affiliated Hosp 1, 17 Yongwai Main St,Nanjing West Rd, Nanchang 330006, Jiangxi, Peoples R China

来源：

LWT-FOOD SCIENCE AND TECHNOLOGY | 2020年 / 130卷

基金：

中国国家自然科学基金;

关键词：

Monascus; Genistein; Metabolomics; Citrinin; UHPLC-Q-TOF-; MS/MS; FLIGHT MASS-SPECTROMETRY; BIOSYNTHESIS; METABOLISM; PATHWAY; GENE;

D O I：

10.1016/j.lwt.2020.109613

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Monascus mold produces useful bioactive compounds such as Monascus pigments, monacolin K, and ergosterol. In this study, metabolomics analysis was used to investigate the metabolic profile changes in Monascus aurantiacus Li AS3.4384 (MALA) induced by genistein at different fermentation times using UHPLC-Q-TOF-MS/MS. The addition of genistein was found to not only reduce citrinin but also suppress significant differential metabolites. A total of 21 significant differential metabolites were involved in the most significant metabolic pathways (impact >0.1). These metabolites affected the aspartic acid, glutamic acid pathway, tricarboxylic acid (TCA) cycle, pyruvate synthesis pathway, and lysine degradation pathway. Thus, the addition of genistein may change the precursor for citrinin synthesis of Monascus. These findings provide significant insight into the Monascus metabolic pathways affected by genistein and will help to improve the quality control and safety of Monascus in food and supplements.

引用

页数：8

共 31 条

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