Pluripotent stem cells as a source of osteoblasts for bone tissue regeneration

被引：35

作者：

Zhu, Hui ^{[1
]}

Kimura, Takaharu ^{[1
]}

Swami, Srilatha ^{[1
]}

Wu, Joy Y. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Div Endocrinol, Stanford, CA 94305 USA

来源：

BIOMATERIALS | 2019年 / 196卷

基金：

美国国家卫生研究院;

关键词：

Embryonic stem cells; Induced pluripotent stem cells; Osteoblasts; Differentiation; Tissue engineering; IN-VITRO DIFFERENTIATION; GENE-EXPRESSION ANALYSIS; MARROW STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; DIRECTED DIFFERENTIATION; MESENCHYMAL PROGENITORS; SERUM-FREE; VIVO; LINEAGE; CHONDROCYTES;

D O I：

10.1016/j.biomaterials.2018.02.009

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Appropriate and abundant sources of bone-forming osteoblasts are essential for bone tissue engineering. Pluripotent stem cells can self-renew and thereby offer a potentially unlimited supply of osteoblasts, a significant advantage over other cell sources. We generated mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) from transgenic mice expressing rat 2.3 kb type I collagen promoter-driven green fluorescent protein (Col2.3GFP), a reporter of the osteoblast lineage. We demonstrated that Col2.3GFP ESCs and iPSCs can be successfully differentiated to osteoblast lineage cells that express Col2.3GFP in vitro. We harvested GFP(+) osteoblasts differentiated from ESCs. Genome wide gene expression profiles validated that ESC- and iPSC-derived osteoblasts resemble calvarial osteoblasts, and that Col2.3GFP expression serves as a marker for mature osteoblasts. Our results confirm the cell identity of ESC- and iPSC-derived osteoblasts and highlight the potential of pluripotent stem cells as a source of osteoblasts for regenerative medicine. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：31 / 45

页数：15

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