A 5′-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1

被引：36

作者：

Whiley, DM ^{[1
]}

Sloots, TP

机构：

[1] Royal Childrens Hosp & Hlth Serv Dist, Sir Albert Sakzewski Virus Res Ctr, Clin Virol Res Unit, Herston, Qld 4029, Australia

[2] Univ Queensland, Clin Med Virol Ctr, Brisbane, Qld 4072, Australia

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2005年 / 53卷 / 04期

关键词：

influenza; PCR; real-time; H5N1;

D O I：

10.1016/j.diagmicrobio.2005.08.002

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A 5'-nuclease real-time reverse transcriptase-polymerase chain reaction assay was developed for the detection of influenza type A and was validated using a range of influenza A subtypes, including avian strains, and 126 nasopharyngeal aspirate samples. The results show the assay is suitable for screening for influenza A infections, particularly in regions where avian strains may be circulating. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：335 / 337

页数：3

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