Self-assembly of DNA Origami Using Rolling Circle Amplification Based DNA Nanoribbons

被引：21

作者：

Liu, Bing ^{[1
]}

Ouyang, Xiangyuan ^{[2
]}

Chao, Jie ^{[2
]}

Liu, Huajie ^{[2
]}

Zhao, Yun ^{[1
]}

Fan, Chunhai ^{[2
]}

机构：

[1] Sichuan Univ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China

来源：

CHINESE JOURNAL OF CHEMISTRY | 2014年 / 32卷 / 02期

基金：

中国国家自然科学基金;

关键词：

self-assembly; DNA origami; rolling circle amplification; nanoribbons; NANOSCALE SHAPES; FOLDING DNA; ARRAYS;

D O I：

10.1002/cjoc.201300827

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

During the development of structural DNA nanotechnology, the emerging of scaffolded DNA origami is marvelous. It utilizes DNA double helix inherent specificity of Watson-Crick base pairing and structural features to create self-assembling structures at the nanometer scale exhibiting the addressable character. However, the assembly of DNA origami is disorderly and unpredictable. Herein, we present a novel strategy to assemble the DNA origami using rolling circle amplification based DNA nanoribbons as the linkers. Firstly, long single-stranded DNA from Rolling Circle Amplification is annealed with several staples to form kinds of DNA nanoribbons with overhangs. Subsequently, the rectangle origami is formed with overhanged staple strands at any edge that would hybridize with the DNA nanoribbons. By mixing them up, we illustrate the one-dimensional even two-dimensional assembly of DNA origami with good orientation.

引用

页码：137 / 141

页数：5

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