Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts

被引：10
作者：
Choresca, Casiano H., Jr. [2 ]
Koo, Ok Jae [1 ]
Oh, Hyun Ju [1 ]
Hong, So Gun [1 ]
Gomez, Dennis K. [3 ]
Kim, Ji Hyung [2 ]
Lee, Byeong Chun [1 ]
Park, Se Chang [2 ]
机构：
[1] Seoul Natl Univ, Coll Vet Med, Dept Theriogenol & Biotechnol, Seoul 151742, South Korea
[2] Seoul Natl Univ, Coll Vet Med, Lab Aquat Anim Med, Seoul 151742, South Korea
[3] Seoul Natl Univ, Coll Vet Med, Brain Korea 21 Program Vet Sci, Seoul 151742, South Korea
来源：
CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 01期
关键词：
Cell cycle synchronization; G0/G1; phase; Goldfish; Serum starvation; Culture to confluence; Roscovitine; MEDAKA ORYZIAS-LATIPES; ADULT SOMATIC-CELLS; NUCLEAR TRANSFER; SKIN FIBROBLASTS; SERUM STARVATION; ENUCLEATED EGGS; KINASE-ACTIVITY; ROSCOVITINE; CALVES; FISH;
D O I：
10.1016/j.cellbi.2008.09.015
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 mu M) induce cell cycle arrest at the G0/G1 phase. Crown Copyright (C) 2008 Published by Elsevier Ltd on behalf of International Federation for Cell Biology. All rights reserved.
引用
收藏
页码：65 / 70
页数：6
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