Extraction and characterization of collagen hydrolysates from the skin of Rana chensinensis

被引：17
|
作者：
Zhao, Yuanyuan [1 ]
Wang, Zhanyong [1 ]
Zhang, Jing [1 ]
Su, Tingting [1 ]
机构：
[1] Liaoning Shihua Univ, Coll Chem Chem Engn & Environm Engn, Fushun 113001, Peoples R China
来源：
3 BIOTECH | 2018年 / 8卷
关键词：
Rana chensinensis; Skin; Collagen hydrolysates; Extraction; Characterization; PEPSIN-SOLUBILIZED COLLAGENS; PHYSICOCHEMICAL CHARACTERISTICS; SOLUBLE COLLAGEN; I COLLAGEN; ACID; SPECTROSCOPY; CAPACITY; SCALES;
D O I：
10.1007/s13205-018-1198-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The production of Hasma generates plentiful non-edible by-products in China and Central Asia. As one of main by-products, the skin of Rana chensinensis is discarded as waste without utilization. In this work, R. chensinensis skin collagen (RCSC) hydrolysates were extracted using pepsin under acidic conditions. The yield of RCSC is 15.1% (w/w). Amino acid analysis revealed that RCSC contained glycine (204.5/1000 residues) and imino acids (182/1000 residues). RCSC exhibited high solubility in acidic pH (1-4) and low NaCl concentrations (< 2%, w/v). Differential scanning calorimetry indicated that the denaturation temperature of RCSC was 33.5 degrees C. Scanning electron microscopy analysis confirmed their well-defined fibril morphologies. The results indicated that the skin of R. chensinensis is an alternative source of collagen hydrolysates, and RCSC can serve as a potential source applying in foodstuff and medical industry.
引用
收藏
页数：8
相关论文
共 50 条
  • [31] Collagen Extraction from Animal Skin
    Matinong, Andrea Marie E.
    Chisti, Yusuf
    Pickering, Kim L.
    Haverkamp, Richard G.
    BIOLOGY-BASEL, 2022, 11 (06):
  • [32] Molecular Cloning of Novel Antimicrobial Peptide Genes from the Skin of the Chinese Brown Frog, Rana chensinensis
    Zhao, Jie
    Sun, Yan
    Li, Zhi
    Su, Qi
    ZOOLOGICAL SCIENCE, 2011, 28 (02) : 112 - 117
  • [33] Extraction of collagen from pig skin
    Jin, Y.
    Xu, S.
    Liu, Z.
    Wei, D.
    Jingxi Huagong/Fine Chemicals, 2001, 18 (05): : 302 - 304
  • [34] Studies on Rana tigerina skin collagen
    Sai, KP
    Babu, M
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2001, 128 (01): : 81 - 90
  • [35] Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities
    Valcarcel, Jesus
    Fraguas, Javier
    Hermida-Merino, Carolina
    Hermida-Merino, Daniel
    Pineiro, Manuel M.
    Vazquez, Jose Antonio
    MARINE DRUGS, 2021, 19 (09)
  • [36] Ultrasonic-assisted Extraction and Structure Characterization of Collagen from Camel Skin
    Shi R.
    He J.
    Jirimutu
    Journal of Chinese Institute of Food Science and Technology, 2022, 22 (02): : 213 - 223
  • [37] Molecular Cloning of cDNAs Encoding Antimicrobial Peptide Precursors from the Skin of the Chinese Brown Frog, Rana chensinensis
    Shang, Dejing
    Yu, Fenghui
    Li, Junfeng
    Zheng, Junjie
    Zhang, Lifang
    Li, Yang
    ZOOLOGICAL SCIENCE, 2009, 26 (03) : 220 - 226
  • [38] Antioxidant Activity of Collagen Hydrolysates from Fish Skin with a Microbial Collagenase
    Baehaki, Ace
    Suhartono, Maggy T.
    Sukarno
    Syah, Dahrul
    Setyahadi, Siswa
    RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES, 2016, 7 (02): : 1677 - 1682
  • [39] FUNCTIONAL PROPERTIES OF CATFISH SKIN COLLAGEN HYDROLYSATES
    Ayat, A. A.
    Shakir, K. A.
    IRAQI JOURNAL OF AGRICULTURAL SCIENCES, 2021, 52 (06): : 1528 - 1540
  • [40] Rana Chensinensis Skin Collagen/Poly(lactic-co-glycolic acid) Microspheres as a Potential Antibiotic Controlled Delivery System by Coaxial Electrospray
    Wang, Yilong
    Sun, Dahui
    Dong, Wenying
    Ma, Wendi
    Zhang, Mei
    JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, 2018, 8 (11) : 1588 - 1595
12345