Synthesis of biomimetic oxygen-carrying compartmentalized microparticles using flow lithography

被引：28

作者：

An, Harry Z. ^{[1
]}

Safai, Eric R. ^{[1
]}

Eral, H. Burak ^{[1
]}

Doyle, Patrick S. ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

来源：

LAB ON A CHIP | 2013年 / 13卷 / 24期

基金：

美国国家科学基金会;

关键词：

EMULSIONS; DELIVERY; ENCAPSULATION; MICROGELS; SHAPE; NANOEMULSIONS; MONODISPERSE; FABRICATION; PARTICLES; TRANSPORT;

D O I：

10.1039/c3lc50610j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a microfluidic approach for lithographically photo-patterning compartmentalized microparticles with any 2D-extruded shape, down to the cellular length scale (similar to 10 microns). The prepolymer solution consists of a UV crosslinkable perfluorodecalin-in-water nanoemulsion stabilized by Pluronic (R) F-68. The nanoemulsions are generated using high-pressure homogenization and are osmotically stabilized by the trapped species method. The presence of PFC droplets increases the solubility and diffusivity of oxygen in the prepolymer solution, thereby enhancing the rate of O-2 inhibition during microparticle synthesis. We develop a simple model that successfully predicts the augmented O-2 mass transport, which agrees well with experimental data. Informed by our analytical results, cell-sized composite microgels are generated by controlling the oxygen environment around the polydimethylsiloxane (PDMS) microfluidic synthesis device. These nanoemulsion composites are functionally similar to red blood cells as oxygen carriers. Such bio-inspired polymeric particles with controlled physical properties are promising vehicles for drug delivery and clinical diagnostics.

引用

页码：4765 / 4774

页数：10

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